Intestinal flora isolated culturing device

Abstract

The invention relates to an in vitro culture device, which can simulate intestinal flora for intestinal and continuous flow, comprising a temperature control system of a fermentation tank, an automatic fresh culture medium feeding and a waste liquid output system and a safeguard system for an environment required by the growth of the intestinal flora; the fermentation tank that is connected with a constant temperature water bath cauldron and a micro submersible and is provided with a water bath clamp ferrule is designed; the culture medium can continuously flow into while the waste liquid can continuously flow out the system through the connection of a peristaltic pump; an anaerobic environment, pH scope and nutrient conditions are reached by inputting nitrogen and a pH controller. The human intestinal flora culture device successfully constructed in the invention is characterized by low cost, easy use and simple operation. Besides, the human intestinal flora can grow stably upon inspection and the comparability of the structure of the intestinal flora is up to 80 percent or more compared with the intestinal flora before the culture. Therefore, the invention is applicable to the safety evaluation and research of the influence to the human intestinal flora caused by antibacterial residues, and the invention is also applicable to the function evaluation of nutrient substances and function and safety evaluation and research of medicines and foods.

Description

technical field [0001] The invention relates to an in vitro culture device for bacterial flora, in particular to an in vitro culture device for intestinal flora capable of simulating the intestinal tract and continuously flowing, and is especially suitable for studying and observing the influence of foreign medicine or food on the intestinal flora. Background technique [0002] Gut flora is a balanced ecosystem that plays a very important role in maintaining the health of humans and animals. While inhibiting the growth of pathogenic bacteria, antibacterial drugs can also affect the normal intestinal flora, disrupt its balance, and cause superinfection. In recent years, studies have found that antibacterial drugs remaining in animal foods can also have adverse effects on the intestinal flora of the human body, and some functional foods such as probiotics can regulate the intestinal flora of humans and animals. Therefore, the safety or functional evaluation of drugs and funct...

AI Technical Summary

Problems solved by technology

The key part of the experimental device is the fermenter, which has automatic heating, automatic stirring, automatic flow rate control, etc., but the equipment is not only expensive, but also has some defects in design, such as the electric heating tube directly heats the culture content, and the Growth of certain temperature-sensitive microorganisms has adverse effects, uneven agitation of fermentation contents, etc.

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