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Method for extracting active ingredient of natural leech essence

A natural hirudin and active ingredient technology, applied in the direction of leech inhibitors, leech/worm medical raw materials, protease inhibitors, etc., can solve the problems of low effective active ingredients, unsuitable for industrial production, high impurity content, etc. , to achieve the effect of being conducive to production management, huge scientific research and market development prospects, and low impurity content

Inactive Publication Date: 2008-01-23
滕海英 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But above-mentioned method also has a lot of deficiencies, after main extracting, effective active ingredient is not high, and impurity content is high, is not suitable for injection medicine, and the extraction process of some methods is slower, and the method that has is not suitable for industrialized production, so the extraction method of hirudin at present Many people are still researching, trying to find an extraction method with high efficiency, low cost and high active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Firstly, 10 kilograms of blood-sucking pheasant leeches are cleaned with purified water, and then 20 kilograms of 20% ethanol aqueous solution (diluted with water for medical alcohol) is added to the total mass of the leeches, soaked for 50 hours, and filtered to obtain filtrate 1 and medicine residue; The philippines leeches medicine residues are minced, and then 3 times the amount of the medicine residues is added with a 20% acetone aqueous solution (pure acetone can be diluted with water) and soaked. After 35 hours, it is filtered to obtain filtrate 2; filtrate 1 and filtrate 2 are respectively After the ethanol and acetone are vacuum recovered, the filtrate is combined and freeze-dried in a conventional vacuum to obtain the extract of the phenanthrene hirudin polypeptide freeze-dried powder suitable for medical use.

Embodiment 2

[0034] Firstly, 10 kilograms of blood-sucking phoenix leeches are cleaned with distilled water, and 30 kilograms of 25% ethanol are added to soak for 30 hours according to the total mass of the leech, and then filtered to obtain filtrate 1 and medicine residue; the phoenix leeches medicine residue is minced, Then add 4 times the amount of dregs to the acetone aqueous solution with a purity of 25%. After 30 hours, filter to obtain filtrate 2. After the filtrate 1 and filtrate 2 are vacuumed to recover ethanol and acetone, the filtrate is combined, and the filtrate is conventionally vacuum freeze-dried. The extract material of the freeze-dried powder of phenanthrene hirudin polypeptide suitable for medical use is obtained.

Embodiment 3

[0036] Firstly, 10 kilograms of blood-sucking pheasant leeches are cleaned with purified water, and then 28 kilograms of 15% ethanol aqueous solution is added to the total mass of the leech, soaked for 40 hours, and then filtered to obtain filtrate 1 and medicine residue; Crush it and add 3 times the amount of dregs to the 25% acetone aqueous solution. After 38 hours, filter to obtain filtrate 2. After the filtrate 1 and filtrate 2 are vacuumed to recover ethanol and acetone, the filtrate is combined, and the filtrate is conventionally vacuum frozen. After drying, the natural hirudin (ie phenanthrene hirudin) polypeptide freeze-dried powder extract material suitable for medical use is obtained.

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Abstract

The invention discloses an extract method of the effective constituent of the Natural hirudin. The method is that the blood-sucking leech is washed cleanly by water and is added with the ethanol water with content of 15 per cent to 25 per cent to soak for 30h to 50h, and then is filtered to gain the filter liquor and dregs of a decoction; the Poecilobdella manillensis waste residue is broken into pieces and is added with aqueous acetone solution with content of 15 per cent to 25 per cent to soak, and then is filtered to gain the filter liquor; after recovering ethanol and acetone from the two-time filter liquor in the vacuum, the filter liquor is combined and frozen and dried in the vacuum conventionally to gain the product. The invention is simple in process, low in cost, higher in yield, good in product quality and long in retention period. The ''three wastes'' are not discharged in the whole production process, and the product can work as the raw material in such three fields as medicine, hairdressing and health food.

Description

Technical field [0001] The present invention relates to a method for extracting hirudin, in particular to a method for extracting effective components of natural hirudin from blood-sucking leeches and hirudos. Background technique [0002] Leech is the general name of Hirrdinea in the Annelida phylum (Annelida) in the classification of animals, commonly known as Leech, and the original English text is [Leech]. There are more than 500 species known in the world, divided into 4 orders. There are 2 orders, 8 families, 33 genera and nearly 100 species in my country. Among the species that suck the blood of mammals are Hirudinidae (Hirudinidae) and Poecilobdeaal, and Haemadipsidae (Haemadipsidae) Haemadipsa. [0003] The modern research on the chemical composition of blood-sucking leeches began in the late 1950s. By the mid-1980s, with the development of biomedicine, foreign scholars had performed amino acid sequencing and molecular cloning of blood-sucking leeches, which made the res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/815A61K35/62
Inventor 覃裕永
Owner 滕海英
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