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Method for producing arachidonic acid with fresh water green algae cultivation by photoautotroph mode

A technology of arachidonic acid and freshwater green algae, applied in the direction of unicellular algae, fermentation, etc., can solve the problem of low content of arachidonic acid, and achieve the effect of solving the problem of harvesting

Inactive Publication Date: 2008-01-02
天津禄升海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Before the application of the present invention, there have been many reports and patents on the production of arachidonic acid, such as patents CN1175956, CN1255545C, CN1323904, CN1362522, CN15399825, CN1537378, etc., but they are all limited to cultivating and producing arachidonic acid by heterotrophic fermentation. Because the content of arachidonic acid in general microalgae is very low, it is still blank to use photoautotrophic method to cultivate green algae on a large scale and commercially produce arachidonic acid.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Put the sterilized fresh water into the pipeline photobioreactor of the first-level breeding, adjust the pH to 6-8, and the nutrient concentration to 1.5g / liter NaNO 3 , 36mg / L CaCl2, 20mg / L Na2CO3, 75mg / L MgSO4, 30mg / L K2HPO4, 1mg / L Fe-EDTA and trace element solution, inoculated according to 0.1g biomass / L, and washed into the solution containing 1-5% The filtered air of carbon dioxide makes the algae liquid circulate in the closed pipe. Breeding for 5-10 days; entering the second-level breeding stage, place the above-mentioned culture culture solution in the shallow waterway culture pond in the glass greenhouse, use natural light source for culture, and inoculate the culture solution from the above-mentioned first-level photobioreactor at an inoculation density of 0.1 g biomass / liter, cultured for 5-10 days; entering the third-level breeding stage, according to the density of 0.1g biomass / L, transfer the above-mentioned secondary seed expansion liquid to the above-men...

Embodiment 2

[0047] According to the steps described in Example 1, wherein the conditions for using the culture medium are: temperature at 25°C, pH value 6-8, 0.25g / L NaNO 3 , 2.5mg / L CaCl2, 25mg / L NaCl, 75mg / L MgSO4, 25mg / L K2HPO4, 12.5mg / LFeSO4-EDTA and trace element solution.

Embodiment 3

[0049] According to the steps described in Example 1, the conditions for using the culture medium are: the temperature is 15°C, the pH value is 6-8, and the nutrient concentration is 1.0 / liter NaNO 3 , 2.5mg / L CaCl2, 25mg / L NaCl, 75mg / L MgSO4, 25mg / L K2HPO4, 12.5mg / L FeSO4-EDTA and trace element solution.

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Abstract

The invention relates to a technology for culturing and producing microalgae in particular to a method for producing arachidonic acid (tetracosenoic acid)by culturing fresh water algae with photoautotroph method. The technical project comprises the following steps: (1)domesticating and screening green algae seed; (2)culturing the domesticated green algae seed in the culture medium with photo biological reactor; (3)accumulating the quick growth of the algae cell and polyunsaturated fatty acid; proceeding with a large scale culture of the green algae; (4)collecting when the biomass in the culture liquid and the arachidonic acid in the algae reach the requirements; (5)getting arachidonic acid by collecting the algae cell; (6)circulating the collected breeding waste water. The invention is used to produce arachidonic acid.

Description

technical field [0001] The invention relates to microalgae culture and production technology, in particular to a method for cultivating freshwater green algae to produce long arachidonic acid (eicosatetraenoic acid) in a photoautotrophic manner. Background technique [0002] The green alga Neochloris or Parietochloris is a single-celled algae belonging to the Chlorophyta. The cells are round or oval, with a diameter of 10-15um. It is up to 20% of the dry weight, and most of the arachidonic acid in the algae is distributed in the oil droplets in the algae, which is consistent with the distribution of the arachidonic acid in the milk, and it is easy to be absorbed and utilized by the human body. Arachidonic acid is an important unsaturated fatty acid and the precursor of many eicosanoids, such as prostaglandins, thrombin and hemotriene, etc., and has important physiological functions. Docosatetraenoic acid is also an important component of cells and cell membranes, and plays ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/40
Inventor 陈幕钊高清潭
Owner 天津禄升海洋生物科技有限公司
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