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Bee venom refining process

A purification method and technology for bee venom, which are applied to medical preparations containing active ingredients, pharmaceutical formulas, unknown raw materials, etc., can solve the problems of complicated purification and purification process, limited bee venom, and refined products can not meet application requirements, etc. scientifically sound effects

Inactive Publication Date: 2007-12-19
张文礼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far the clinical application of bee venom is still very limited, the main reason is that there are many components of bee venom, the refining and purification process is complicated, and the cost is high; the refined products still cannot meet the application requirements of clinical and other special fields, especially the preparation of electrophoresis Technical requirements of grade melittin

Method used

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  • Bee venom refining process

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Embodiment

[0011] Take 100g of crude bee venom, add 500ml of 95% ethanol to make a suspension, shake for 4 hours, filter to remove insoluble impurities such as bee sting needles; remove the liquid phase after the filtrate is centrifuged (3000r / min) for 20min, and add 300ml of absolute ethanol to the precipitate Dehydrate, then extract the proposed precipitate with 300ml of PH=6.4 distilled water at 10±2°C for 12 to 15 hours, discard the precipitate, and precipitate the extract with ethanol to remove the aqueous ethanol solution; add 6000ml of 0.15 molar hydrogen to the precipitate Dissolve in ammonium and 4000ml n-butanol solution, shake for 2 hours to separate the liquid from the solid, discard the precipitate, distill the extract to recover n-butanol, freeze-dry the concentrate to obtain refined bee venom.

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Abstract

The bee venom refining process includes the following steps: dissolving coarse bee venom in alcohol to form suspension, filtering to eliminate propolis, honey and other impurity, centrifuging the filtrate and eliminating liquid phase, dewatering the precipitate with anhydrous alcohol, leaching the precipitate in water at 10+ / -2 deg.c for 12-15 hr, precipitating the leached liquid with alcohol, eliminating water solution of alcohol, extracting the precipitate in ammonium hydroxide and n-butanol solution, distilling the extracted liquid, concentrating and freeze drying to obtain refined bee venom. The refined bee venom may be used in different clinical research and application fields, including preparing electrophoresis level melittin.

Description

technical field [0001] The invention relates to a method for refining honey bee venom, in particular to refining the bee venom by using the refined bee venom to extract melittin. Background technique [0002] Bee Venom (bee venom, referred to as bee venom) is produced by the bee venom and stored in the poison sac. When the bee is stimulated, it is a kind of venom discharged from the stinger. Bee venom is a highly complex mixture of pharmacological and biochemical activities Substances are widely used in medicine. But so far the clinical application of bee venom is still very limited, the main reason is that there are many components of bee venom, the refining and purification process is complicated, and the cost is high; the refined products still cannot meet the application requirements of clinical and other special fields, especially the preparation of electrophoresis Technical requirements for grade melittin. Contents of the invention [0003] The object of the presen...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61K35/644
Inventor 张文礼孙井元
Owner 张文礼
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