Chinese chestnut wine and preparation method thereof Chinese chestnut fungus root preparations and preparation method thereof
A technology of chestnut fungi and preparations, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of long seedling breeding cycle, poor absorption capacity, weak healing ability, etc., to improve growth level, enhance Absorptive capacity and the effect of small wilting coefficient
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0027] 1. Selection of strains: Shanxi Rhizopus edulis and Boletus edulis isolated from the chestnut forest in Huairou Bohai Sea and the ectomycorrhizal fungus Rhizopus genus (Rhizopogon) symbiotic with chestnuts were selected.
[0028] 2. Strain culture:
[0029] 1) Activation of strains: a small amount of hyphae were picked from the strains Rhizogenus Shanxi and Boletus cerevisiae cultured on the inclined plane with a sterilized inoculation needle and connected to the PDA plate medium for activation. Activation conditions: 25°C, dark culture for 3 days, when the hyphae grow to 1 / 3 of the diameter of the petri dish. Wherein the PDA medium is: 200g of potatoes, 20g of glucose, 18g of agar, and 1000ml of distilled water.
[0030] 2) Liquid fermentation culture: pick a small amount of activated hyphae from the plate medium with a sterilized inoculation needle, and inoculate them into a liquid culture bottle, and the medium is PDA or ODA. Culture conditions: temperature 25°C, p...
Embodiment 2
[0035] 1. Selection of strains: choose Rhizopus edulis and Boletus edulis isolated from the chestnut forest in Huairou Bohai Sea and the ectomycorrhizal fungus Rhizopus genus (Rhizopogon) symbiotic with chestnuts.
[0036] 2. Strain culture:
[0037] 1) Activation of strains: use a sterilized inoculation needle to pick a small amount of mycelia from the strains of Rhizobia rubrum and Boletus cerevisiae cultured on a slant and connect them to the PDA plate medium for activation. Activation conditions: 25°C, dark culture for 5 days, when the hyphae grow to 1 / 2 of the diameter of the petri dish. Wherein the PDA medium is: 150g of potatoes, 25g of glucose, 20g of agar, and 1000ml of distilled water.
[0038] 2) Liquid fermentation culture: pick a small amount of activated hyphae from the plate medium with a sterilized inoculation needle, and inoculate them into a liquid culture bottle, and the medium is PDA or ODA. Culture conditions: temperature 25°C, pH about 5, shaking cultur...
Embodiment 3
[0043] 1. Selection of strains: select the ectomycorrhizal fungus Rhizopogon and Boletus edulis isolated from chestnut forests in Huairou Bohai Sea and symbiotic with chestnuts.
[0044] 2. Strain culture:
[0045] 1) Activation of strains: use a sterilized inoculation needle to pick a small amount of hyphae from the strains of Rhizobia nigeri and Boletus cerevisiae cultured on an inclined plane and connect them to the PDA plate medium for activation. Activation conditions: 25°C, dark culture for 4 days, when the hyphae grow to 1 / 3 of the diameter of the petri dish. Wherein the PDA medium is: 250g of potatoes, 15g of glucose, 15g of agar, and 1000ml of distilled water.
[0046]2) Liquid fermentation culture: pick a small amount of activated hyphae from the plate medium with a sterilized inoculation needle, and inoculate them into a liquid culture bottle, and the medium is PDA or ODA. Training conditions:
[0047] The temperature is 25°C, the pH is about 5, and the shaking c...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com