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Tissue engineering venous valve and its production

A technology of tissue engineering and venous valves, applied in the fields of heart valves, medical science, prostheses, etc.

Inactive Publication Date: 2007-11-21
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the joint application of bone marrow-derived MAPC and EPC as seed cells for tissue engineering valves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Construction of canine tissue engineered venous valve in vitro

[0026] Experimental animals, materials and reagents:

[0027] Hybrid dog (female, 6 months old, body weight 15kg), purchased from Shanghai Naval Medical Research Institute,

[0028] Animal use license number: SRXK (Army) 2002-044.

[0029] M199, DMEM-LG, CD13, bFGF (the above reagents were purchased from Gibco)

[0030] MCDB-201, dexamethasone, ascorbic acid 2-phosphate, ITS, FN, IGF-1, VEGF,

[0031] FITC-secondary antibody, Cy3-secondary antibody (the above reagents were purchased from Sigma)

[0032] EGM-MV2 (purchased from PromoCell company)

[0033] Fetal calf serum (FCS) (purchased from Hyclone company)

[0034] rhEGF, rhPDGF-BB (purchased from R&D System Company)

[0035] rhLIF (purchased from Prospec Company)

[0036] CD44, CD45, CD133, MHC-II, VEGF, VE-cadherin, α-actin, Calponin,

[0037] SM-MHC (the above antibodies were purchased from Santa Cruz Company)

[0038] CD14, CD31,...

Embodiment 2

[0064] Example 2: In vivo transplantation experiment of the prepared canine tissue engineered venous valve

[0065] The prepared tissue-engineered venous valve was transplanted into the left external jugular vein of the recipient dog by end-to-end anastomosis, and a piece of venous valve stent was transplanted into the right external jugular vein as a control.

[0066] 1. Color ultrasound of the external carotid artery was performed 4 weeks after the operation, and it was found that the blood flow on the experimental side was smooth, while the lumen on the control side was narrowed, and the blood flow was thinner and slower than that on the left side.

[0067] 2. The recipient dog was executed, the graft was taken out, and the venous valve on the experimental side was generally observed to be intact, the venous wall was smooth, and no thrombus was seen; the venous wall on the control side was obviously thickened, the lumen was narrowed, and there were several wall-attached thro...

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PUM

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Abstract

The invention is concerned with biology structure process technology field. At present, the foreign research of tissue-engineered venous valvular is on the primary phase, and some problems is not clear and needs to be solved, such as the selection of cell and bracket, growth and adhering of cell and inner process of body. The aim of this invention is to afford tissue-engineered venous valvular replanting substance and the replanting substance is made up of seed cell and bracket. The said seed cell ismultipotent adult progenitor cells (MAPC) andendothelial progenitor cells (EPC), and the said bracket is homogeneity variant acellularized venous with valvular. It also affords the production method of the said tissue-engineered venous valvular. This material is fit for repairing the insufficiency tissue-engineered venous valvular to the deep vein imaging of lower extremity with wide source and without the problem of immunogenicity and ethic. The invention has excellent function and effect on animals.

Description

technical field [0001] The invention relates to the technical field of biological tissue engineering, and relates to a tissue-engineered venous valve and a preparation method thereof. Background technique [0002] Venous valve transplantation is often the last option for primary valvular insufficiency, valve destruction after complete recanalization of thrombosis, and congenital valvular deficiency. However, in addition to the limited source and large damage of autologous venous valve transplantation, there are still problems such as insufficient valve strength and the caliber of the valve-bearing vein is often difficult to meet the requirements. In recent years, the development of cardiovascular tissue engineering technology has made it possible to construct tissue engineered venous valves. Studies have shown that although there are many pairs of valves in each deep vein, only one pair of functional valves can significantly improve the problem of blood reflux, which makes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61F2/24
Inventor 温昱张传森党瑞山李彬
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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