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Cartilage tissue engineering rack and its application

A cartilage tissue and engineering technology, applied in the field of double-layer collagen scaffolds, can solve problems such as adverse reactions, expensive medical polymer materials, rejection reactions and poor long-term effects, and achieve easy availability, good biological performance, biological Excellent academic performance

Active Publication Date: 2007-11-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these treatments have their inherent drawbacks
Such as the adverse reaction of degradation products to the body, the rejection of the body caused by non-degradable materials and poor long-term effects, etc.
[0003] Most of the current applications are polymer materials or collagen sponges and other scaffolds to transfer chondrocytes to repair cartilage defects, but medical polymer materials are expensive and their degradation products are destructive to tissues, and there are many cartilage defects repaired by single-layer collagen sponges. The starting point of the present invention is to find a scaffold with good biological performance, which can provide enough intercommunicated cell tissue accommodation space, and has a blocking effect at the same time.

Method used

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  • Cartilage tissue engineering rack and its application
  • Cartilage tissue engineering rack and its application
  • Cartilage tissue engineering rack and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Tendon collagen extracted by acid extraction method (Schor method can be used: take 10g of fresh rat tail tendon, soak it in 1:1000 bromogeramine solution for 10-15 minutes, take it out, rinse it repeatedly with normal saline for more than 5 times, pour Remove the normal saline and trim it to 1mm with scissors 3 The left and right tissue pieces were put into an Erlenmeyer flask, and 100 ml of 0.05M Tris / HCl (PH7.5) solution containing 1M NaCl was added for pre-extraction to remove non-collagenous impurities. Store in a refrigerator at 4°C for 4 days, and shake regularly 4 to 6 times a day. After 4 days, discard the supernatant, add 400ml of 0.5M acetic acid, extract at 4°C for 4 days, and shake regularly. Then centrifuge at high speed and low temperature for 3 hours (2000g) to remove insoluble fragments, and the supernatant is liquid crude collagen. Add the same amount of 20% NaCl solution to the crude collagen solution, and the collagen precipitates out as white floc...

Embodiment 2

[0021] The double-layer scaffold obtained in Example 1 is compounded with growth factors by physical freeze-drying or chemical cross-linking, and can be compounded with FGF, BMP2, IGF, VEGF, PDGF, etc. individually or separately.

Embodiment 3

[0023] The rabbit cartilage defect was repaired with the double-layer scaffold obtained in Example 1, and the cartilage repaired well (see FIG. 4 ).

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Abstract

The present invention provides one kind of double layer cartilage tissue engineering rack comprising one compact layer and one loose layer communicated each other. The rack has excellent biological performance, sufficient cell tissue holding space, certain blocking effect and capacity to allow nutritious matters to pass through freely. It is used in repairing human body's cartilage tissue and constructing extracorporeal cartilage tissue. It is made through preparing solution of different concentrations with acid extracted collagen, freeze drying to obtain collagen sponge, flattening, injecting collagen solution of different concentration, and drying for the second time to obtain the double layer cartilage tissue engineering rack. The rack has the pore size controlled through controlling the concentration of the solution.

Description

(1) Technical field [0001] The invention relates to a double-layer collagen support for cartilage tissue engineering, in particular to a support for cartilage repair in human body and construction of cartilage tissue engineering in vitro. (2) Background technology [0002] With the improvement of people's health requirements, more and more people advocate sports, and injuries to cartilage, bones, tendons, etc. are more common and more and more. Human articular cartilage is subjected to mechanical forces caused by various activities every day, and the muscle function gradually declines after middle age, which can easily lead to joint damage and cartilage destruction, while the cartilage's self-repair ability is weak, and continuous development will lead to cartilage degeneration. Osteochondritis thus occurs. In China, the prevalence of osteoarthritis is 50% among people over 50 years old, and 80% among people over 75 years old, and 53% of severe cases will lead to loss of jo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/24A61L31/10A61L31/16
Inventor 欧阳宏伟齐义营
Owner ZHEJIANG UNIV
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