Method for measuring human blood serum cetylic acid and oleic acid concentration ratio with gas phase chromatography and application thereof
A technology of gas chromatography and gas chromatograph, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of lack and difficulty in early diagnosis, and achieve the effect of accurate prevention and diagnosis, early diagnosis and auxiliary diagnosis.
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[0024] The present invention will be further described below in conjunction with specific examples, but there is no limitation thereto.
[0025] Sample collection: Serum samples were provided by the Department of Cardiothoracic Surgery, Drum Tower Hospital, Affiliated Hospital of Nanjing University.
[0026] Materials and Reagents:
[0027] Reagents: Chromatographically pure palmitic acid and oleic acid were purchased from Sigma Corporation in the United States, analytically pure concentrated sulfuric acid was purchased from Analytical pure methanol, analytically pure chloroform and anhydrous sodium sulfate were purchased from Tianjin No. 1 Chemical Reagent Factory.
[0028] Equipment: water bath, votex, centrifuge, gas chromatograph HP-6890 (Agilent, USA).
[0029] 1 Preparation of standard products and acquisition of human serum samples
[0030] Preparation of standard products: use chromatographically pure palmitic acid and oleic acid to prepare and dissolve in methanol a...
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