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Clostridium Daqing clostridium and application thereof

A Clostridium and Clostridium technology, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of high oil content, high viscosity, difficult to remove or degrade, etc.

Inactive Publication Date: 2007-10-17
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oilfield sewage containing polyacrylamide is a kind of complex and special sewage, which has the characteristics of high oil content and high viscosity, so it is difficult to remove or degrade polyacrylamide in oilfield sewage

Method used

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  • Clostridium Daqing clostridium and application thereof
  • Clostridium Daqing clostridium and application thereof
  • Clostridium Daqing clostridium and application thereof

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Experimental program
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specific Embodiment approach 1

[0009] Specific Embodiment 1: In this embodiment, Clostridium Daqing-WL (Clostridium Daqing WL) is identified as a new species of Clostridium (Clostridium) - Clostridium Daqing, and is preserved in China Microorganism Culture Preservation Management Committee General Microbiology Center, the preservation date is January 10, 2007, and the preservation number is CGMCC No.1911; Clostridium daqing-WL is a Gram-positive bacillus, endophytic spores, facultative It grows under the condition of 10~65℃, the length of the strain is variable, the width is 0.4μm~0.8μm, the length is 1.4μm~4μm, without flagella; Clostridium daqing-WL grows as a black round colony on the solid medium, the surface Smooth, neat edges; Clostridium daqing-WL can use polyacrylamide as the only carbon source.

[0010] The Clostridium genus Clostridium daqing-WL in this embodiment has different lengths, the strain width is 0.4 μm-0.8 μm, and the length is 1.4 μm-4 μm. Figure 1 is a transmission electron microscop...

specific Embodiment approach 2

[0012] Specific embodiment two: present embodiment Clostridium genus Clostridium Daqing-WL (Clostridium Daqing WL) bacterial strain is to measure by following steps:

[0013] (1) Isolation, purification and screening of Clostridium clostridium Daqing-WL strain:

[0014] a. Take 5-10 mL of the aqueous solution of the mother liquor tank of the polyacrylamide injection station and put it into an anaerobic bottle filled with nitrogen and containing anaerobic sterile water, and add 5-20 glass beads and shake it under the condition of 30-38°C 4 ±0.1h;

[0015] b. Dilute the bacterial solution with anaerobic sterile water, then inoculate the bacterial solution in the improved Starkey solid medium, and cultivate it with a "rolling tube" for 7 to 10 days;

[0016] c. Carry out multiple isolation and purification until it is confirmed as a pure strain by electron microscopy;

[0017] (2) Selection of highly efficient degrading bacterial strains: after the pure bacterial strains are en...

specific Embodiment approach 3

[0029] Embodiment 3: In this embodiment, the Clostridium genus Clostridium daqing-WL can use polyacrylamide as the sole carbon source, and can be used to biodegrade polyacrylamide.

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Abstract

The present invention relates to clostridium 'daqing' clostridium and its uses. The present invention solves the problem it is difficult to remove or degrade polypropylene of oil field sewage. The clostridium 'daqing' clostridium -WL is preserved at CGMCC on January 10, 2007, preserving number being CGMCC No. 1911. The 'daqing' clostridium is Gram-positive bacilli, with endo-spore, facultative anaerobic, grows under pH 5.0-11.0,10-65 degree. The strain is variable in length, breadth being 0.4um-0.8um, length being 1.4um-4um, free of flagellum. The inventive 'daqing' clostridium -WL is a new strain, and can use polypropylene as unique carbon source which can be used in polypropylene biodegradation. 10ml 'daqing' clostridium -WL of concentration 6x10<6> per ml is added in oil field sewage with polypropylene concentration being 500mg / L and the polypropylene degradation rate is 35%-76% after 20 days.

Description

technical field [0001] The present invention relates to a kind of microorganism and its application. Background technique [0002] Water-soluble polymer polyacrylamide (HPAM) has been widely used in tertiary oil recovery in oilfields. In Daqing Oilfield in China, "three-element compound flooding" oil recovery technology is widely used for tertiary oil recovery. "three-element" mainly includes ultra-high molecular weight polyacrylamide ( HPAM), alkalis and surfactants. Ultra-high molecular weight polyacrylamide (HPAM) aqueous solution is used as an oil displacement agent to inject into the oil layer, so that the mobility ratio between crude oil with high viscosity (mainly saturated hydrocarbons, aromatics, colloids and asphaltenes) and water decreases. , Weaken the viscous fingering of water, thereby enhancing oil recovery. The produced fluid of polymer flooding not only contains crude oil, but also contains a large amount of polyacrylamide, resulting in a large increase in...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F1/58C02F3/34C12R1/145
Inventor 魏利马放
Owner HARBIN INST OF TECH
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