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Method for measuring all-core antigen of hepatitis C virus

A technology of hepatitis C virus and core antigen, which is applied in the field of detection of total core antigen of hepatitis C virus, can solve the problems of reduced sensitivity and achieve the effects of increased detection rate, accelerated cleavage, and high specificity

Inactive Publication Date: 2007-08-15
湖南景达生物工程有限公司
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Problems solved by technology

[0005] However, once the antibody is produced in the body, an antigen-antibody complex can be formed between the anti-core antigen antibody and HCV-cAg after seroconversion, and the sensitivity of its detection is greatly reduced.

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  • Method for measuring all-core antigen of hepatitis C virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Comparison of the results of three detection methods: free core antigen assay, total core antigen assay and PCR assay.

[0016] The present invention involves a total of 75 serum samples, among which 50 samples are outpatient cases of Xiangya Hospital of Central South University, patients with single transaminase elevation or clinical suspicion of liver disease, 18 samples are outpatient cases of Beijing You'an Hospital, and 7 samples are outpatient cases of Yueyang, Hunan Province Outpatient cases of the Second Municipal Hospital.

[0017] 1. Use the hepatitis C virus core antigen enzyme-linked immunosorbent assay kit, perform the enzyme-linked immunosorbent reaction according to the kit operation steps (see the application document of the publication number CN1877330), measure the OD value of each well with a microplate reader at a wavelength of 450nm, and the critical value is The average value of the two negative control values ​​is +0.06, and the OD valu...

Embodiment 2

[0025] Example 2: Comparison of the results of total core antigen assay and quantitative fluorescent PCR assay

[0026] The total core antigen assay and conventional quantitative fluorescent PCR method were used. The 50 serum samples involved were from Xiangya School of Medicine, Central South University, and they were judged as positive by the copy number > 0.5×103. Quantitative fluorescent PCR detected 24 positive samples. The total There were 16 positive specimens tested for antigen, and the results are shown in Table 2, where "+" means positive and "-" means negative. The results showed that the fluorescent quantitative PCR could detect the virus infection when the virus copy number was >106 and the core total antigen could be detected.

[0027] Among the 50 cases, none of them were positive by ELISA but negative by RT-PCR or quantitative PCR, which shows that the detection reagent for total core antigen has high specificity, and the positive rate is close to that of PCR d...

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Abstract

This invention discloses one method to test hepatitis C virus total nuclear antigen, which hcomprises the following steps: testing front protein transformation agent and non-ion surface activity agent to pre-process this sample; then adopting hepatitis C to test the nuclear antigen enzyme immune dialogue case. This invention pre-process can impact Ig molecule transformation area especially long area to reduce its combination opportunity with antigen to promote antigen compound isolation and to make the nuclear antigen fully exposed.

Description

technical field [0001] The invention relates to the technical field of virus antigen detection, in particular to a detection method for hepatitis C virus total core antigen. Background technique [0002] After hepatitis C virus (HCV) infects the human body, nearly half of the people will develop chronic hepatitis, and some of them will eventually develop into liver cirrhosis and liver cancer. So far, there is no vaccine to prevent hepatitis C, and there is no specific drug for treatment, so early diagnosis is still an effective means to prevent HCV transmission. [0003] The detection of hepatitis C virus (HCV) is of great significance for the early diagnosis of hepatitis C and the guidance of clinical treatment. There is a long period of positive viremia and negative serum antibodies after human infection with HCV, which is called test serum. The "window period" before the yang transition period. During this period, HCV replicates actively and is highly infectious. Medica...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/569
Inventor 刘俊王志耘欧阳兰香
Owner 湖南景达生物工程有限公司
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