Building method of colorectal cancer neostomy orthotopic transplantation model

A method for establishing a colorectal cancer technology, applied in the field of establishing biological feature models, can solve the problems of large use of animals and inconvenient observation of molecular pathological changes of tumors, etc., and achieve the effect of reducing the amount of use and easy establishment.

Inactive Publication Date: 2007-08-15
NANJING HOSPITAL OF T C M
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Problems solved by technology

However, due to the deep location of the colon, it is not very convenient to observe the morphology of the tumor after orthotopic transplantation and the molecular pathological changes of the tumor. Only after the experimental animals are killed can the changes in the intervention results be observed, and the amount of animals used is relatively large.

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  • Building method of colorectal cancer neostomy orthotopic transplantation model

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Embodiment Construction

[0013] Steps for establishing the stoma orthotopic transplantation model of colorectal cancer of the present invention:

[0014] Colostomy in nude mice: Intraperitoneal injection of ketamine (0.17mg) anesthetized the nude mice, disinfected the left abdomen with tincture of iodine, cut a small mouthful of skin and peritoneum, raised the gaseum of the nude mice by about 0.5cm from the abdominal wall, and connected the cecum and cecum with 5-0 suture. The surrounding skin was sutured and fixed. After the operation, pay attention to supplementing the test subjects with glucose saline to strengthen nutrition. The stoma site will scab in 2 to 3 days after the operation, and the scab will fall off in about a week, exposing the intestinal tract. The next step can be performed two weeks after the stoma.

[0015] Orthotopic tumor transplantation at the stoma: Inoculate 50 mg of mouse tumor subcutaneously, make a cell suspension in 1 ml of normal saline, and inoculate 0.2 ml under the st...

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Abstract

The invention provides a method for establishing orthotopic transplantation model of colorectal cancer, which comprises a) selecting and culturing colon adenocarcinoma cell line and preparing cell suspension; b) performing colostomy on living experimental animal; c) and inoculating the above colon adenocarcinoma cell suspension to submucosal stroma to allow natural growth. The biological traits such as route of metastasis of the transplanted adenocarcinoma cells are similar to that of the primary colon adenocarcinoma cell. The invention is advantageous in that the model is easy to be established, the transplanted colon adenocarcinoma locates at body surface so that it can be sampled and observed conveniently to meet the requirement for dynamic research, and the consumption of experimental animals can be reduced.

Description

technical field [0001] The invention relates to a method for establishing a biological characteristic model, especially an animal transplantation model of colorectal cancer, and the application of the method in the study of tumor biological characteristics. Background technique [0002] Colorectal cancer models play an important role in colorectal cancer research. An ideal animal model of colorectal cancer should have the following characteristics: conform to the biological characteristics of colorectal cancer, especially the metastasis pathway of the tumor should be consistent with colorectal cancer; the tumor is stable and can stably reflect the characteristics of the primary tumor; it is easy to observe and obtain materials ; Easy to make. At present, the common animal models of colorectal cancer include subcutaneous inoculation model, colon orthotopic transplantation model and transgenic model. The subcutaneous inoculation model is widely used in colorectal cancer rese...

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Application Information

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IPC IPC(8): A61K49/00
Inventor 金黑鹰刘秀芳丁义江刘飞倪敏丁曙晴叶辉
Owner NANJING HOSPITAL OF T C M
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