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Aminoglutaric acid fermentation production method for secondary inoculation superimposing bioepiderm

A technology of secondary inoculation and production methods, applied in the biological field, to achieve the effects of reduced production costs, coordinated growth of acid production levels, and increased tank volume

Inactive Publication Date: 2007-08-08
GUANGDONG IND TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to solve the problems existing in the existing glutamic acid fermentation production, to provide a kind of glutamic acid that utilizes glucose as a raw material, uses biotin-deficient glutamic acid producing strains as production strains, and superimposes biotin through secondary inoculation. Fermentation production method, increase glutamic acid fermentation single-tank output, reduce production cost, and obtain significant economic benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 - at 80m 3 Implementation on the fermenter (biotin concentration 5.0μg / L superimposed 2.0μg / L)

[0024] Production strain: (Brevibacterium tianjinese S9114, selected by Yun Fenglin and Zhou Wanbing of South China University of Technology, has been widely used in production)

[0025] Seed tank: 1.0m 3 Universal fermenter

[0026] Fermentation tank: 80m 3 Universal fermenter

[0027] The cultivation of the first batch of seeds: glucose 18kg, urea 3.5kg, KH 2 PO 4 1.1kg, MgSO 4 ·7H 2 O 0.5kg, molasses 20kg, corn steep liquor 30kg, FeSO 4 and MnSO 4 1.4g each, 0.15kg defoamer, adjust the pH to 7.0 with lye, set the volume to 700L, sterilize the real tank to 121-122°C and keep it warm for 10min, and add 1000mL shake flask seeds when cooled to 31-32°C. During the cultivation process, the stirring speed is 250-300r / min, the temperature is controlled at 30-33°C, the ventilation rate is controlled at 0.20-0.28vvm (relative to the volume of the seed liquid) a...

Embodiment 2

[0030] Example 2 - at 120m 3 Implementation on the fermenter (biotin concentration 12μg / L superimposed 5.0μg / L)

[0031] Production strain: (Brevibacterium tianjinese FM 84-415 , selected by Zheng Shanliang, Fudan University, has been widely used in production)

[0032] Seed tank: 20m 3 Universal fermenter (for the first batch of seed culture), 10m 3 Universal fermenter (for the second batch of seed culture)

[0033] Fermentation tank: 120m 3 Universal fermenter

[0034] Cultivation of the first batch of seeds: Glucose 700kg, KH 2 PO 4 26kg, MgSO 4 ·7H 2 O 9.6kg, molasses 150kg, corn steep liquor 400kg, biotin (reagent pure) 200mg, FeSO 4 and MnSO 4 Each 24g, defoamer 3.0kg, constant volume 12000L, sterilize the solid tank to 121-122°C and keep warm for 10min, when cooled to 30-32°C, adjust the pH to 7.0 with liquid ammonia, and insert 1200mL shake flask seeds. During the cultivation process, the temperature is controlled at 31-32°C, the nitrogen source is supplem...

Embodiment 3

[0037] Example 3 - at 150m 3 Implementation on the fermenter (biotin concentration 7.0μg / L superimposed 2.5μg / L)

[0038] Production strain: (Brevibacterium tianjinese S9114, selected by Yun Fenglin and Zhou Wanbing of South China University of Technology, has been widely used in production)

[0039] Seed tank: 10m 3 Universal fermenter

[0040] Fermentation tank: 150m 3 Universal fermenter

[0041] The cultivation of the first batch of seeds: glucose 190kg, urea 38kg, KH 2 PO 4 12kg, MgSO 4 ·7H 2 O 6kg, molasses 60kg, corn steep liquor 250kg, biotin (reagent pure) 150mg, FeSO 4 and MnSO 4 15g each, 1.2kg defoamer, adjust the pH to 7.0 with lye, set the volume to 7500L, sterilize the real tank to 121-122°C and keep it warm for 10min, and add 400mL shake flask seeds when cooled to 30-32°C. During the cultivation process, the stirring speed was 160r / min, the temperature was controlled at 31-33°C, the ventilation rate was controlled at 0.10-0.28vvm (relative to the vol...

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PUM

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Abstract

The invention discloses a manufacturing method of fermented glutamic acid of secondary seeded superimposed biotin, which comprises the following steps: allocating ferment culture medium; setting the sum of biotin quantity and the ferment liquid of first batch of seed at 5.0-12.0mug / L corresponding to initial bulk; sterilizing; cooling; seeding the first batch of mature seed liquid; stirring; fermenting 9-18h; seeding the second batch of mature seed liquid; carrying biotin through the second batch of seed liquid into ferment tank; setting the biotin quantity of second batch of seed liquid at 2.0-5.0mug / L corresponding to ferment liquid; aerating gas continuously to ferment 28-36h.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically refers to a glutamic acid fermentation production method using glucose as a raw material, using a biotin-deficient glutamic acid producing strain as a production strain, and superimposing biotin through secondary inoculation. Background technique [0002] At present, most of the domestic glutamic acid fermentation production uses biotin-deficient strains, and the biotin "super sub-appropriate" fermentation process has a higher acid production level than the traditional biotin "sub-appropriate" fermentation process. However, the biotin "super-sub-appropriate" fermentation process has a higher bacterial concentration, which requires a higher dissolved oxygen concentration. Due to the limitation of the oxygen dissolved efficiency of the fermenter, many factories are using the biotin "super-sub-appropriate" fermentation process. Sometimes, the acid production level and the sugar-acid con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14C12R1/13C12R1/15
Inventor 邓毛程梁世中王瑶朱明军朱晓立宋炜
Owner GUANGDONG IND TECHN COLLEGE
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