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Method of preparing monoclonal antibody against human soluble mesothelin related protein

A monoclonal antibody and related protein technology, applied in the direction of anti-animal/human immunoglobulin, biochemical equipment and methods, microorganisms, etc., can solve the problem of no enzyme-linked immunosorbent assay kit that can detect soluble mesothelin

Inactive Publication Date: 2007-08-08
吴小华
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  • Abstract
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Problems solved by technology

But so far, there is still no ELISA kit that can detect soluble mesothelin

Method used

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  • Method of preparing monoclonal antibody against human soluble mesothelin related protein
  • Method of preparing monoclonal antibody against human soluble mesothelin related protein
  • Method of preparing monoclonal antibody against human soluble mesothelin related protein

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Embodiment Construction

[0093] The present invention will be further described below in conjunction with the examples, but not as a further limitation to the present invention.

[0094] The preparation method of anti-human soluble mesothelin-related protein monoclonal antibody comprises the following process steps:

[0095] A. Multi-parameter prediction of human mesothelin B cell epitope and synthesis of antigenic peptide

[0096] (1) Single parameter prediction

[0097] The full length of the amino acid sequence of mesothelin was analyzed by using GOLDKEY software, and the single parameter of the epitope was analyzed by Hopp & Woods hydrophilicity parameters, Welling antigenicity parameters, accessibility parameters, and secondary structure. Prediction, the peaks above the threshold value are the predicted positions; the full length of the amino acid sequence of mesothelin is as follows:

[0098] 1 malptarpll gscgtpalgs llfllfslgw vqpsrtlage tgqeaapldg vlanppniss

[0099] 61 lsprqllgfp caevsglste...

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Abstract

The invention discloses a making method of relative protein mono-cloning antibody of anti-human soluble mesothelium, which is characterized by the following: predicting multi-parameter on the antigen surface position of human mesothelium B; making anti-human SMR mono-cloning antibody; making the monomer-cloning antibody (2H10 and 6D9) of relative protein; possessing higher specificity.

Description

technical field [0001] The invention belongs to the preparation of antibodies, in particular to a preparation method of an anti-human soluble mesothelin-related protein monoclonal antibody. Background technique [0002] Mesothelin (MSLN) is a cell surface glycoprotein with a molecular weight of about 40kD, which is highly expressed in malignant tumors such as mesothelioma, ovarian cancer, and pancreatic cancer. Mesothelin cDNA encodes a precursor protein with a molecular weight of 69kD, which is hydrolyzed into 40kD and 31kD fragments by protease after glycosylation, and the 40kD fragment is connected to the cell membrane through glycosylphosphatidylinositol, called mesothelial The 31kD fragment is called megakaryocyte colony-stimulating factor (megakaryocyte-potentiating factor). Mesothelin includes two isoforms, among which isoform 2 retains the intron between the 16th and 17th exons, so that its protein translation is terminated prematurely, and it is shed from the cell ...

Claims

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Application Information

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IPC IPC(8): C07K16/18C12N5/18
Inventor 吴小华单保恩陈书成杨波孙东霞刘雅坤陈兴
Owner 吴小华
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