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Seashore paspalum young spike isolated culture strain-reproducing technique

A technology of in vitro culture and young spikes, which is applied in the field of plant tissue culture, can solve the problems of unsuitable somatic mutant screening gene transformation, loss of ability of green seedling differentiation, etc., to maintain high-frequency plant regeneration ability and increase induction frequency Effect

Inactive Publication Date: 2007-08-08
新昌县白云草业研究所 +2
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Problems solved by technology

[0005] The technical problem to be solved and the technical task proposed by the present invention are to overcome the translucent, moist, viscous embryogenic callus induced by the existing sea paspalum in vitro culture technology, which loses the ability of green seedling differentiation in the process of subculture , not suitable for somatic cell mutant screening and the defect of Agrobacterium-mediated gene transformation, a plant regeneration technology for in vitro cultured plants of sea paspalum young ears is provided, in order to use the young ears of sea paspalum as explant material, Regulate the osmotic pressure of exogenous plant hormones and medium, obtain a high proportion of granular embryogenic callus, and establish a technical system for high-frequency plant regeneration

Method used

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  • Seashore paspalum young spike isolated culture strain-reproducing technique

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Embodiment Construction

[0025] 1. Materials and Methods

[0026] 1.1 Test material

[0027] The sea paspalum variety "Adalay" (Paspalum vaginatum Sw.cv.Adalay) introduced from the United States was provided by Shenzhen Mission Hills Golf Course as the test material.

[0028] 1.2 Medium

[0029] The basal medium consists of MS macroelements, MS trace elements (Murashige T, Skoog F.Arevised medium from rapid growth and bioassays with tobacco tissuecultures[J].Physiol plant, 1962,15:473~497) and B5 vitamins (GamborgOL, Miller R A, Ojima K. Nutrient requirements of suspension cultures of soybean root cells [J]. Exp. Cell Res., 1968.50: 151-158), a commonly used medium in plant tissue culture, sucrose 30g / L;

[0030] Callus induction medium and callus subculture medium additional hormone: 2,4-dichlorophenoxyacetic acid (abbreviated as 2,4-D, Shanghai Chemical Reagent No. 4 Factory) 2.0mg / L, 6-benzylamino Purine (referred to as BAP, Shanghai Xueman Biotechnology Co., Ltd.) 0.05mg / L;

[0031] Callus dif...

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PUM

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Abstract

The invention relates to a method for cultivating the barnyard grass in vitro. The invention uses the spike in blooming period as the explant, to be cultivated in the callus cultivate medium which contains 2.omg / L of 2, 4-fenclofenac, and 0.05mg / L of 6-benzyl adenine to obtain yellow dry particle callus whose number is more than 40% higher than the induce method; then cultivates in the cultivate medium whose agar-solidifier content is improved one time to generate, while the callus is particle structure, via three generations, the green differentiation can reach 98%. The invention can be used to cultivate barnyard grass, or the like.

Description

technical field [0001] The invention discloses a plant regeneration technology for isolated culturing of sea paspalum young panicles, which relates to a culture technology for acceptor materials suitable for micropropagation, screening of somatic cell mutants and gene transformation, and belongs to plant tissue culture methods. technical background [0002] Sea paspalum (Paspalum vaginatum Sw.) has high resistance to salt, drought, waterlogging and abrasion. As a lawn grass, it is widely planted in tropical and subtropical regions of the world. The rising stars of warm-season grasses such as Pseudomonas chinensis (Xie Xinming, Lu Xiaoliang. The excellent characteristics and utilization value of paspalum germplasm resources [J], Journal of South China Agricultural University, 2004, 25 (Supplement II): 64~ 67). Sea paspalum is mainly distributed in Taiwan, Yunnan, Guangdong and Hainan provinces in my country. The results of many years of introduction experiments showed that ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 梁流芳佘建明杨郁文董民强吴瑛瑛钟小仙陈志一倪万潮
Owner 新昌县白云草业研究所
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