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Antigen epitope and mutant of lyase in gamma bacteriophage of anthrax bacillus, and application

A technology of phage lysing enzyme, Bacillus anthracis, application in preventive and/or therapeutic medicine, diagnostic field

Inactive Publication Date: 2010-06-23
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the wild-type γ-phage lyase PlyG has certain immunogenicity. Antibodies cross-reacting with PlyG can be detected by Western Blot in animal and human serum, and anti-PlyG antibodies can neutralize PlyG to a certain extent. lyase activity, thus limiting the clinical application of PlyG

Method used

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  • Antigen epitope and mutant of lyase in gamma bacteriophage of anthrax bacillus, and application
  • Antigen epitope and mutant of lyase in gamma bacteriophage of anthrax bacillus, and application
  • Antigen epitope and mutant of lyase in gamma bacteriophage of anthrax bacillus, and application

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Experimental program
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Embodiment 1

[0044] Embodiment 1, the acquisition of Bacillus anthracis gamma phage lyase mutant MPlyG

[0045] 1. Preparation of anti-PlyG antibody

[0046] Get 0.5mg of purified PlyG (for its prokaryotic expression and purification method, see the patent application of application number: 200610057298.5, the title of the invention is "a method for expressing Bacillus anthracis gamma phage lyase and its special gene"), and compare it with complete Freund's The adjuvant was mixed at a volume ratio of 1:1 to immunize rabbits, and immunized once every 10 days. After 35 days, blood was collected from the heart to obtain antiserum, and then the antiserum was used to detect whether anti-PlyG antibody was produced in the serum by Western Blot method. Test results such as figure 1 As shown, it shows that the anti-PlyG antibody that can specifically bind to PlyG is produced in the serum.

[0047] 2. Construction of Escherichia coli expression vector containing PlyG deletion mutant gene

[0048]...

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Abstract

This invention discloses Bacillus anthracis gamma-phase lyase epitope, its lyase mutant and application. This invention provides the epitope of Bacillus anthracis gamma-phase lyase PlyG, and mutant lyase MPlyG. The epitope is 164th-172nd amino acid residues of lyase PlyG, and is shown in SEQ ID No.4. Mutant lyase MPlyG is derived from lyase PlyG by deleting one or more of 164th-172nd amino acid residues. Animal experiments show that compared with PlyG, MPlyG has lower antigenicity, lower immunogenicity, and similar lyase activity. Therefore, MPlyG can be used to prevent and treat charbon and manufacture corresponding drugs.

Description

technical field [0001] The present invention relates to an antigenic epitope of Bacillus anthracis γ-phage lyase and its mutant and its application, in particular to an antigenic epitope of Bacillus anthracis γ-phage lyase and its mutant in the diagnosis, prevention and / or preparation of anthrax application in therapeutic drugs. Background technique [0002] Anthrax is a zoonotic acute infectious disease caused by Bacillus anthracis. The main susceptible animals are sheep, cattle, horses and other herbivorous livestock. The blood of dead animals that die of anthrax does not coagulate, and often discharges a large amount of blood with natural channels. Germs. Due to the different routes of infection, anthrax is clinically divided into skin type, gastrointestinal type and inhalation infection type. Cutaneous anthrax is more common, mainly manifested as skin anthrax pain in exposed parts; gastrointestinal anthrax is relatively rare, in addition to fever and gastrointestinal s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/195C12N15/31C12N9/88C12N15/60C12N15/09C12N15/63A61K38/51A61P31/04
Inventor 曹诚杨尧马清钧
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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