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Liquid enzyme agent for quick detection of residual pesticide toxicity and preparation method and using method thereof

A technology of pesticide residues and liquid enzymes, applied in biochemical equipment and methods, measurement/inspection of microorganisms, and analysis through chemical reactions of materials to achieve the effect of ensuring accuracy

Inactive Publication Date: 2009-09-09
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The liquid enzyme reagent for rapid detection of organophosphorus and carbamate pesticide residues of the present invention can be stored stably at room temperature at 20-25°C, thereby overcoming the shortcomings of currently used enzymes and solving the problem of current organophosphorus and carbamate pesticides Existing problems in rapid detection of residues,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Put 30μL of acetylcholinesterase, 2mL of 0.1M Tris-HCl buffer solution with pH=7.8—8.2, and 5mg of sodium taurine into a beaker and stir evenly to make a liquid enzyme reagent; put 0.1mL of liquid enzyme reagent into a 2mL safe Put it in a beaker bottle, vacuum it and seal it; usually store it in a refrigerator at 4°C.

[0030] When rapid detection of organophosphorus and carbamate pesticide residues is required, the reagent package is prepared according to the above-mentioned method, and the detection is carried out by the enzyme-catalyzed kinetic photometry measurement with the pesticide residue rapid detector.

Embodiment 2

[0032] Put 100μL of butyrylcholinesterase, 10mL of 0.1M phosphate buffer solution with pH=7.8—8.2, and 20mg of sodium taurine into a beaker and stir evenly to make a liquid enzyme reagent; put 0.3mL of the liquid enzyme reagent into Put it in a 2mL ampoule bottle, vacuum it and seal it; usually store it in a refrigerator at 4°C.

[0033] When rapid detection of organophosphorus and carbamate pesticide residues is required, the reagent package is prepared according to the above-mentioned method, and the detection is carried out by enzyme-catalyzed kinetic photometry with a pesticide residue rapid tester

Embodiment 3

[0035] Put 50μL of acetylcholinesterase, 4mL of 0.1M Tris-HCl buffer solution with pH=7.8—8.2, and 8mg of sodium taurine into a beaker and stir evenly to make a liquid enzyme reagent; put 0.2mL of the liquid enzyme reagent into a 2mL safe Put it in a beaker bottle, vacuum it and seal it; usually store it in a refrigerator at 4°C.

[0036] When rapid detection of organophosphorus and carbamate pesticide residues is required, prepare a reagent pack according to the above method, and perform detection by color change.

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PUM

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Abstract

The invention provides a liquid enzyme reagent for rapid detection of organophosphorus and carbamate pesticide residues, a preparation method and a use method thereof. The components of the liquid enzyme reagent for rapid detection of organophosphorus and carbamate pesticide residues in the invention include: cholinesterase; buffer solution; and stabilizer. The preparation method of the present invention: 1) prepare a liquid enzyme reagent; 2) put the liquid enzyme reagent into an ampoule bottle, vacuumize it and seal it, and store it in a refrigerator at 4° C. at ordinary times. The liquid enzyme reagent of the present invention can be stored stably for more than 15 days at room temperature (20-25° C.), while keeping the loss of enzyme activity no more than 10%. Therefore, it can be used in conjunction with substrate reagent, chromogen reagent and buffer solution reagent to meet the The application of on-site rapid analysis of pesticide residues in vegetables, fruits, environmental water or grain, and to ensure the accuracy of test results.

Description

technical field [0001] The invention relates to a composition, in particular to a liquid composition reagent, in particular to an enzyme-containing liquid composition reagent for rapid detection of organophosphorus and carbamate pesticide residues. Background technique [0002] The harm of residual pesticides to human health is one of the hot spots in food safety issues. Worldwide, poisoning incidents caused by excessive residues of organophosphorus and carbamate pesticides have occurred frequently, of which 69.5% are caused by organophosphorus caused by pesticides. Therefore, it is very important to monitor pesticide residues in food and environment. At present, the enzyme inhibition method is usually used for the rapid detection of organophosphorus and carbamate pesticide residues, and the degree of enzyme inhibition is used to measure by enzyme-catalyzed kinetic photometry with the aid of a pesticide residue rapid tester, or by color change, or by acidity measurement an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78C12Q1/00
Inventor 邹明强王大宁张晓华邱月明王楠郭飞马
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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