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Quick diagnosis method for brown stem rot of broccoli

A rapid diagnosis and broccoli technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as economic losses, delay prevention, and wrong diagnosis of broccoli brown stem disease, and achieve simple use, simple and rapid application, and economical avoidance. effect of loss

Inactive Publication Date: 2009-05-13
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in the winter of 2002, about 100 mu of broccoli brown stem disease in the Qianjiang Reclamation Farm in Xiaoshan District, Hangzhou City, Zhejiang Province was misdiagnosed as a nutritional deficiency; disease, resulting in wrong diagnosis of broccoli brown stem disease, delaying effective prevention and control, resulting in a large amount of economic losses

Method used

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  • Quick diagnosis method for brown stem rot of broccoli
  • Quick diagnosis method for brown stem rot of broccoli

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: (indoor microtome section)

[0021] (1) field sampling: randomly collect broccoli curds in the field where broccoli is produced;

[0022] (2) Slicing with an indoor slicer: Take the flower stems of the broccoli flower bulbs collected as standard samples, or the flower stems of the flower bulbs, cut them into small cubes with length×width×height of 5mm×5mm×5mm, place them on a slicer, and perform Longitudinal (from the epidermal tissue to the pith tissue) and / or transverse slices, the thickness of the slices can be 0.01mm or less than 0.01mm, and healthy broccoli stem tissue is used as a control;

[0023] (3) Microscopic observation: place the slices under a 100-fold microscope for microscopic examination and microphotography;

[0024] (4) Diagnosis: Under the microscope, the unique pathogen structure of broccoli brown stem disease - the haustoria can be observed, which is the broccoli brown stem disease caused by Peronospora parasitica Var. brassica (see ...

Embodiment 2

[0025] Embodiment 2: (manual sectioning in the field)

[0026] (1) Field sampling: Broccoli bulbs were randomly collected in the field where broccoli is produced.

[0027] (2) Manual sectioning in the field: Take the flower stems of the collected broccoli curd standard samples, or the pedicels of the flower bulbs, and use a manual blade directly in the field to slice longitudinally (from epidermal tissue to pith tissue) and / or transversely, and slice The thickness can be 0.01mm or less than 0.01mm, and the stem tissue of healthy broccoli is used as the control.

[0028] (3) Microscopic observation: the slices were placed under a microscope for microscopic observation.

[0029] (4) Diagnosis: If the unique pathogen structure of broccoli brown stem disease - haustoria can be observed under the microscope, it is the broccoli brown stem disease caused by Peronospora parasiticaVar. "Then it is a normal plant or a nutrient deficient plant.

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Abstract

The invention discloses a method for detecting the brown stem rot illness of blue and white vegetable, which is formed by sampling in field, slicing, watching with microscope, and diagnosing. The invention can only watch if there is pathogeny organization in the cut film when in the step of watching with microscope, the absorber can make the diagnosis the if it is belong to the brown stem rot illness. The invention has simple device, high speed and reliable diagnose.

Description

technical field [0001] The invention relates to the technical field of diagnosis of vegetable diseases, in particular to a rapid diagnosis method for broccoli brown stem disease. Background technique [0002] Broccoli (broccoli), also known as broccoli and green cauliflower, is a variety of Brassica oleracea in the family Cruciferae (Cruciferae) that produces green or purple curd bulbs. It is a one-year or biennial herb with the scientific name Brassica oleracea L.var .italica Planch, chromosome number 2n=2x=18. Evolved from cabbage, the evolution center is the eastern Mediterranean coast. It is eaten with green or purple flower balls. Because of its good flavor, high nutritional value, bright green and crisp texture, and refreshing and delicious flavor, it is deeply loved by consumers and is one of the top five best-selling vegetables in the international market. Although its cultivation history is relatively short, it has developed rapidly and is widely planted in countr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/06G01N33/00
Inventor 王汉荣王连平茹水江方丽赵建阳
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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