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Method for obtaining Brassica oleracea var. acephala DC. small spore regenerated plants and special culture medium

A technology for regenerating plants and rooting medium, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of low efficiency, difficult to capture recessive traits, and long time, and achieve broad application prospects and adaptability wide, high embryonic rate

Inactive Publication Date: 2008-10-01
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because cabbage plants are biennial plants, they need long-term and strict low-temperature vernalization treatment to flower, and it takes 8-10 years to obtain homozygous materials by repeated selfing in traditional breeding, which is not only time-consuming, but also inefficient , and it is difficult to capture recessive traits with ornamental value
So far, there have been no reports at home and abroad about the free microspore culture technology of kale and its application in breeding.

Method used

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  • Method for obtaining Brassica oleracea var. acephala DC. small spore regenerated plants and special culture medium
  • Method for obtaining Brassica oleracea var. acephala DC. small spore regenerated plants and special culture medium
  • Method for obtaining Brassica oleracea var. acephala DC. small spore regenerated plants and special culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the cultivation of kale microspore regeneration plant

[0029] The donor mother plants of ornamental kale (Brassica oleracea var. acephala) were grown in plastic greenhouses with insect-proof nets. The genotypes covered various types of ornamental kale, and the leaf types included whole round leaves, wavy leaves, crepe leaves, lobed leaves and deeply lobed leaves, etc., the leaf colors include white, light yellow, pink, bright red, purple red, etc., a total of 15 materials, in the first ten days of March to June (Note: During the entire collection season, remove in time Bloom and pod branch and weak branch, guarantee the exuberant growth of new inflorescence) collect microspore and carry out in vitro culture with the method of the present invention, concrete process comprises the following steps:

[0030] 1) Free microspore culture

[0031] DAPI fluorescent staining by nucleus (Colemen A W, Goff L J. Application offluorochrome to pollen biology I. Mithram...

Embodiment 2

[0039] Embodiment 2, the cultivation of kale microspore regeneration plant

[0040] The donor mother plants of ornamental kale (Brassica oleracea var. acephala) were grown in plastic greenhouses with insect-proof nets. The genotypes covered various types of ornamental kale, and the leaf types included whole round leaves, wavy Leaves, crepe leaves, lobed leaves and deeply lobed leaves, etc., leaf color includes white, pink, scarlet, purple red and light yellow, etc., a total of 16 parts of materials, collected microspores in the first ten days of March to June and carried out in vitro with the method of the present invention Cultivation, the specific process includes the following steps:

[0041] 1) Free microspore culture

[0042] Through the DAPI fluorescent staining of the nucleus, microscopic examination, determine the development period of the microspore, get the flower bud containing 80% mononuclear marginal stage microspore cell and 20% binucleate stage cell, the flower...

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Abstract

The invention relates to a method for obtaining collard regenerated plant and special cultivation base. Wherein, said method comprises (1), planting the collard into induced cultivation base, until the density is 5*10<4-5*10<5n / mL, at 30-35Deg.C and in dark, treating it at high temperature for 24-72hours, and under 24-26Deg. C and in dark, inducing idiosome; (2), when the idiosome has been formed, under 24-26Deg. C, 1500-2500Lux, cultivating for 5-10days while each day lights 14-18hours; then grafting the seed into differentiation cultivation base, to be cultivated in same conditions; (3) when grows out branch and leaf, transplanting it into rooting cultivation base, under 24-26Deg. C, 1500-2500Lux, lighting 14-18hours each day, to root, to obtain the collard regenerated plant.

Description

technical field [0001] The invention relates to a plant microspore culture method and a special medium thereof, in particular to a method for obtaining kale microspore regenerated plants and a special medium thereof. Background technique [0002] Plants are heterosis, so current crop breeding focuses on cross breeding. In this way, on the one hand, the growth advantage of the hybrid can be used to ensure high yield and disease resistance of the crop variety. On the other hand, due to the complex genetic background of the hybrid, it is not suitable to save the seed for use, thereby protecting the interests of the hybrid developer. In crossbreeding, the genetic background of the parents used must be homozygous, and hybrids with the same traits can be obtained after mating. In order to obtain the homozygous parent material of the genetic background, in many crops, it is necessary to artificially assist self-pollination, and it will take 6-10 years to achieve relative homozygos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 刘凡张月云赵泓
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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