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Method of extracting avenabeta glucosan

A technology for glucan and oat, applied in the field of extracting oat beta-glucan, can solve the problems of high cost, difficult removal, low product purity and the like, and achieve the effects of improved product quality, simple extraction and separation method, and high product purity

Active Publication Date: 2008-06-11
北京三友汇智生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods all need to introduce organic solvents, which are costly and difficult to remove; moreover, the method of isoelectric point precipitation is commonly used in the process of separating proteins, and the removal efficiency is low, and the removal process requires centrifugation, which is not conducive to industrial production; these extraction methods still have steps It is cumbersome, many reagents are added, and the purity of the obtained product is not high.

Method used

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  • Method of extracting avenabeta glucosan
  • Method of extracting avenabeta glucosan
  • Method of extracting avenabeta glucosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, extracting β-glucan

[0019] The process flow chart of extracting β-glucan is as follows: figure 1 As shown, the specific process is as follows:

[0020] (1) Selection of oat bran: oat bran between 20 mesh and 100 mesh is selected, and the content of soluble β-glucan is 8.6%.

[0021] (2) β-glucan extraction: add deionized water to the reaction vessel, adjust the pH value to about 8-10, heat to 50-60°C, add oat bran, the weight ratio of oat bran to water is 1:20 , stir evenly for 1-3 hours.

[0022] (3) Protein removal: after extraction, the pH value in the reaction vessel is about 8.0-8.5, add alkaline protease with an activity unit of 1 million U / g (use 5g alkaline protease per 100g oat bran), and continue at 50°C Stir for 1-2 hours.

[0023] (4) Starch removal: add α-amylase with an activity unit of 4000U / g (use 2g of alkaline protease per 100g of oat bran), stir at 80°C for 0.5 hours, and decompose the starch into small molecular weight oligosaccha...

Embodiment 2

[0028] Example 2, functional experiment of oat β-glucan product

[0029] 1. Moisturizing effect experiment

[0030] Using the human body test method, use a moisture tester to test the moisture content and TEWL value of the skin, and select 20 volunteers; the volunteers are half male and half male. Two different emulsions were applied to the skin surface: one containing 5% oat-β-glucan (β-glucan) and one containing 5% hyaluronic acid (HA); tested every half hour for a total of 4 hours. The change curve of the percentage change of the moisture content of the skin surface over time is as follows: figure 2 As shown, the results show that compared with hyaluronic acid, oat β-glucan has a better long-lasting moisturizing effect.

[0031] 2. Oat-β-glucan can increase the content of hyaluronic acid in rat skin

[0032] 2.1 Experimental drugs

[0033] Oat β-glucan used 1% oat β-glucan; the control group used 1% hyaluronic acid.

[0034] 2.2 Experimental animals

[0035] 40 clean...

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Abstract

A process for extracting beta-glucosan from oats includes such steps as mixing oats bran with water, stirring at 50-60 deg.C, regulating pH=8-10, adding proteinase, hydrodyzing the protein in oats bran, adding alpha-amylase, treating at 80 deg.C, filtering to obtain clear liquid, ultrafiltering, and freeze-drying.

Description

technical field [0001] The invention relates to a method for extracting oat beta-glucan. Background technique [0002] Oat β-glucan is a viscous polyglucan linked by β-1,3-, β-1,4-glycosidic bonds, which is mainly concentrated in the aleurone layer of oat bran. Oat β-glucan has multiple functions, such as lowering blood lipids, controlling blood sugar, enhancing immunity, preventing constipation, improving intestinal microenvironment, etc., and is widely used in the fields of medicine and health food. [0003] At present, the preparation of oat β-glucan is generally carried out by solvent extraction or mild alkali extraction. These methods all need to introduce organic solvents, which are costly and difficult to remove; moreover, the method of isoelectric point precipitation is commonly used in the process of separating proteins, and the removal efficiency is low, and the removal process requires centrifugation, which is not conducive to industrial production; these extract...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02
Inventor 董银卯王昌涛王友升兰社益任清赵华何聪芬
Owner 北京三友汇智生物技术有限公司
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