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Extracted product from leaves of podocarpus and preparation method and use

A Luo Han pine and extraction technology, applied in the field of extraction products, can solve problems such as destruction of tissue structure, abnormal secretion of metalloproteinases, abnormal damage to connective tissue, etc.

Active Publication Date: 2007-12-05
TAIYEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Reactive oxygen groups will further damage the tissue structure, and will also deactivate tissue inhibitors of matrix proteases (TIMPs), resulting in abnormal secretion of matrix metalloproteinases in the skin, causing abnormal damage to connective tissue

Method used

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  • Extracted product from leaves of podocarpus and preparation method and use
  • Extracted product from leaves of podocarpus and preparation method and use
  • Extracted product from leaves of podocarpus and preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Embodiment 1. Use water to extract the leaves of Podocarpus macrophyllus (Extraction of the leaves of Podocarpus macrophyllus var. macrophyllus with water)

[0131] At ambient temperature, take about 1 kg of leaves of Podocarpus grandiflora and chop them into pieces, add 10 liters of water and soak for a week. Afterwards, filter with filter paper (Whatman R, No.5), place the obtained filtrate in a water bath, and concentrate under reduced pressure (Eyela SB650, Japan) at about 40°C. The water-extracted product thus obtained (hereinafter referred to as Aq1) was subjected to a tyrosinase activity inhibition test. The % inhibition of tyrosinase activity measured by Aq1 is shown in the table below, where the IC of Aq1 50 About 22mg / mL.

[0132] Table 1. Inhibition % of tyrosinase activity of the extract product "Aq1" of Podocarpus cypress leaves

[0133] 1

Embodiment 2

[0134] Embodiment 2. Use ethanol to extract the leaves of Podocarpus macrophyllus (Extraction of the leaves of Podocarpus macrophyllus var. macrophyllus with ethanol)

[0135] This example is carried out according to the method described in Example 1, but ethanol (95%) is used to extract the leaves of Podocarpus grandiflora, wherein the ratio of leaves to ethanol is maintained at about 10 ml of ethanol for 1 gram of leaves . The ethanol extraction product thus obtained was subjected to a tyrosinase activity inhibition test. The results obtained are shown in Table 2 below, where the deduced IC 50 About 4.4mg / mL.

[0136] Table 2. % inhibition of tyrosinase activity of ethanol extraction products of Podocarpus cypress leaves

[0137] 1

Embodiment 3

[0138] Example 3. Using methanol to extract the leaves of Podocarpus macrophyllus (Extraction of the leaves of Podocarpus macrophyllus var. macrophyllus with methanol)

[0139] This example is carried out according to the method described in Example 1, but 100% methanol is used to extract the leaves of Podocarpus grandiflora, and the ratio of leaves to ethanol is the same as that described in Example 2.

[0140] The methanol-extracted product thus obtained was subjected to a tyrosinase activity inhibition test. The results obtained are shown in Table 3 below, where the deduced IC 50 About 6.0mg / mL.

[0141] Table 3. % Tyrosinase Activity Inhibition of 100% Methanol Extraction Products of Podocarpus grandiflora leaves

[0142] 1

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PUM

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Abstract

This invention relates to a kind of extraction product of podocarp leaves and its preparation method, and its application in skin whiten and rugate-resist. The invention reveals the extraction product obtained form Podocarpus sp. and has the avail of bating the activeness of tyrosinase and MMPs-1, the preparation method of the extraction product and its application in producing skin whitening products. According to this invention can sublime out quercetin and catechin from Podocarpus sp.

Description

technical field [0001] The present invention relates to an extract product of the leaves of Podocarpus species, in particular to an extract obtained from the leaves of Podocarpus sp. and having both the activity of inhibiting tyrosinase and the activity of inhibiting matrix metalloproteinase-1 An effective extraction product, a preparation method of the extraction product and an application of the extraction product in the manufacture of skin whitening and skin anti-wrinkle products. According to the present invention, quercetin and catechin, which have skin whitening and skin anti-wrinkle effects, can be purified from the leaves of Podocarpus sp. Background technique [0002] Skin aging is an inevitable process. Aging skin will produce various changes in tissue morphology and physiological functions, such as skin atrophy, relaxation, loss of elasticity and luster, wrinkles, pigment changes, skin wound healing ability and reduced immunity. In addition to the endogenous agin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/62A61K8/35
Inventor 李美贤郑可大陈世辉吕思洁
Owner TAIYEN BIOTECH
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