Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cytoplasmic transfer to de-differentiate recipient cells

a technology of recipient cells and cytoplasm, which is applied in the direction of non-embryonic pluripotent stem cells, drug compositions, and fused cells, can solve the problems that the cells that form the placenta do not support the nuclear development and the cellular changes cannot be reversed, so as to achieve the effect of preventing senescence or reducing the number of cells

Inactive Publication Date: 2013-04-25
CHAPMAN KAREN B
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent is about a way to prevent cells in tissue culture from becoming old and dying. This is done by introducing the cytoplasm (the liquid part) from a donor cell into a recipient cell. This can help to keep the cells young and healthy.

Problems solved by technology

For example, it was found that trophectodermal cells (the cells that form the placenta) did not support development of the nuclear fusion to the blastocyst stage.
Moreover, it was believed that these cellular changes could not be reversed.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0051]As explained above, the present invention provides novel methods for de-differentiating and / or altering the life-span of desired cells, preferably mammalian cells and, most preferably, human or other primate cells by the introduction of cytoplasm from a more primitive cell type, typically an undifferentiated or substantially undifferentiated cell, e.g., an oocyte or blastomere.

[0052]As noted previously, it was recently reported in the popular press that a group working in the area of artificial insemination and infertility successfully transferred the cytoplasm from the oocyte of a younger woman into that of an older woman and thereby rejuvenated the ability of the older oocyte to be competent for fertilization and embryo development. Based on this anecdotal evidence, coupled with recent papers in the scientific literature which suggest that differentiated adult cells may be effectively “reprogrammed” by nuclear transfer, it was theorized that differentiated cells could be eff...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Genetic stabilityaaaaaaaaaa
sizeaaaaaaaaaa
massaaaaaaaaaa
Login to View More

Abstract

Methods for de-differentiating or altering the life-span of desired “recipient” cells, e.g., human somatic cells, by the introduction of cytoplasm from a more primitive, less differentiated cell type, e.g., oocyte or blastomere are provided. These methods can be used to produce embryonic stem cells and to increase the efficiency of gene therapy by allowing for desired cells to be subjected to multiple genetic modifications without becoming senescent. Such cytoplasm may be fractionated and / or subjected to subtractive hybridization and the active materials (sufficient for de-differentiation) identified and produced by recombinant methods.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation-in-part of International Patent Application No. PCT / US00 / 18063, filed Jun. 30, 2000, which claims priority from U.S. Provisional Application Ser. No. 60 / 141,250, filed Jun. 30, 1999.FIELD OF THE INVENTION[0002]The present invention relates to methods for “de-differentiating” and / or altering the life-span of desired recipient cells, preferably human somatic cells. These methods have application especially in the context of cell therapies and the production of genetically modified cells.BACKGROUND OF THE INVENTION[0003]Nuclear transfer first gained acceptance in the 1960's with amphibian nuclear transplantation. (Diberardino, M. A. 1980, “Genetic stability and modulation of metazoan nuclei transplanted into eggs and ooctyes”, Differentiation, 17-17-30; Diberardino, M. A., N. J. Hoffner and L. D. Etkin, 1984; “Activation of dormant genes in specialized cells”, Science, 224:946-952; Prather, R. S. and Robl, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/873A61K35/12A01K67/027A61K35/54A61K48/00A61P43/00C12N5/074C12N5/10C12N5/16C12N9/12C12N15/07C12N15/09C12N15/12C12N15/16C12N15/18C12N15/19C12N15/79
CPCA61K35/12A61K48/00C12N5/0696C12N5/16C12N9/1241C12N2517/10C12N15/873C12N2503/00C12N2506/00C12N2510/04C12N15/79A61P43/00C12N5/0602C12N5/0606C12N2501/00C12N5/0607C12N2501/727C12N2510/00
Inventor CHAPMAN, KAREN B.
Owner CHAPMAN KAREN B
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com