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Interferon-alpha (IFN-alpha) Fused Protein Having IFN-alpha and Cytoplasmic Transduction Peptide (CTP)

a technology of interferon and alpha, which is applied in the field of interferon (ifn) fused protein having ifn fused to a cytoplasmic transduction peptide (ctp), to achieve the effects of improving penetration and settlement ability, and improving translocation and settlemen

Active Publication Date: 2012-05-31
JW CREAGENE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006]The inventors of the present disclosure have made efforts to develop an interferon with improved translocation and settlement into the liver tissue as well as better effect. As a result, they have prepared a fused protein wherein a cytoplasmic transduction peptide (CTP) is fused to a human interferon (IFN) and confirmed that it has improved translocation, penetration and settlement ability into the liver and thus can solve the side effect and cost problems of the existing therapy.
[0017]The inventors of the present disclosure have made efforts to develop an interferon with improved translocation and settlement into the liver tissue as well as better effect. As a result, they have prepared a fused protein wherein a CTP is fused to a human IFN and confirmed that it has improved translocation, penetration and settlement ability into the liver and thus can solve the side effect and cost problems of the existing therapy.
[0031]In a more specific embodiment of the present disclosure, the PEG bound to the fused protein of the present disclosure has a molecular weight of 10-100 kDa, more specifically 10-60 kDa, and most specifically 15-40 kDa. The PEG having a molecular weight of 10-100 kDa reduces renal clearance and extends residence in blood, thus reducing the administration dose of the fused protein of the present disclosure.
[0037]Typically, the vector of the present disclosure may be established as a cloning vector or an expression vector. And, the vector of the present disclosure may be established using a prokaryotic or eukaryotic cell as host. Specifically, a prokaryotic cell may be used as the host considering that the nucleotide sequence of the present disclosure is derived from a prokaryotic cell and culturing is more convenient.

Problems solved by technology

They are the most severe in the early stage of treatment and usually disappear when the treatment is stopped.

Method used

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  • Interferon-alpha (IFN-alpha) Fused Protein Having IFN-alpha and Cytoplasmic Transduction Peptide (CTP)
  • Interferon-alpha (IFN-alpha) Fused Protein Having IFN-alpha and Cytoplasmic Transduction Peptide (CTP)
  • Interferon-alpha (IFN-alpha) Fused Protein Having IFN-alpha and Cytoplasmic Transduction Peptide (CTP)

Examples

Experimental program
Comparison scheme
Effect test

example 1

Synthesis of Template of Human Interferon-α (IFN-α) Gene for High-Level Expression in E. coli

[0087]Since the Interferon-α (IFN-α) gene originating from human contains a considerable amount of codons that are not expressed well in E. coli, it is known that the degree of expression of IFN protein is very low in the absence of modification. In particular, such codon clusters as AGG / AGA, CUA, AUA, CCA or CCC are reported to degrade both quantity and quality of the proteins expressed in E. coli (2-3).

[0088]Among them, the AGG codon cluster has the worst effect, presumably because the available tRNA pool is limited and the cluster binds competitively with ribosomes due to similarity to the Shine-Dalgarno sequence (4-5).

[0089]Thus, in order to improve the degree of expression, the inventors chemically synthesized the full sequence of the human IFN-α gene excluding the rare codon, in consideration of the codon usage frequency of E. coli. First, in order to obtain the human IFN-α2b gene opt...

example 2

Synthesis of IFN or CTP-fused IFN gene by PCR

[0090]In order to obtain DNA of IFN or IFN with the CTP peptide fused at the N- or C-terminal, SOEing PCR was carried out using the primers shown in Table 2 and using the human IFN genes described in Table 1 as template (6):

TABLE 2SEQPrimerIDnamePrimer sequenceNOCFN-15′- 7TATGGTCGTCGTGCACGTCGTCGTCGTCGTCGTTGCGATCTGCCGCAGACC-3′CFN-25′- 8TAATCTAGAAAAAACCAAGGAGGTAATAACATATGTATGGTCGTCGTGCACGT-3′CFN-35′- 9CAAGGATCCCTCGAGCTATTATTCTTTGCTACGCAGGCT-3′CFN-45′-10GCCTCTAGAAAAAACCAAGGAGGTAATAACATATGTGCGATCTGCCGCAG-3′CFN-55′-11ACGACGACGACGTGCACGACGACCATATTCTTTGCTACGCAGGCT-3′CFN-65′-12TAAGGATCCCTCGAGCTATTAACGACGACGACGACGACGTGCACG-3′

example 2-1

Synthesis of IFN-α Gene (sIFN) Modified for High-Level expression of IFN Protein

[0091]The gene originating from human has the problem in that the level of expression is very low in E. coli in the absence of modification. To solve this problem, the inventors carried out two-step reactions to obtain synthetic gene in consideration of the codon usage of E. coli. First, they mixed the 6 oligomers shown in Table 1, with the same quantity, and allowed them to react at 60° C. for 30 minutes after adding pfu DNA polymerase and dNTP mixture. As a result, a template of human IFN-α gene was synthesized according to the codon usage. Then, PCR was carried out using the template and using the CFN-4 and CFN-3 primers. As a result, the human IFN-α gene was obtained as shown in FIG. 1 (SEQ ID NO: 27). The obtained ˜0.55 kb PCR product is shown as lane 3 in FIG. 4.

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Abstract

Disclosed is an interferon-α (IFN-α) fused protein having IFN-α fused to a cytoplasmic transduction peptide (CTP). The disclosure relates to a fused protein wherein a CTP, which binds well to cell-membrane barriers and enables translocation into the liver, is genetically fused to a human IFN-α, thereby enhancing the conjugation capacity of cell membranes and antiviral activity, inhibiting CTP transport into the cell nucleus, and enhancing the translocation and settlement of the fused protein into the liver and of transduction to the liver tissue. Accordingly, it is possible to develop protein-based medicines effective for preventing or treating various liver diseases associated with viral infection at low doses.

Description

TECHNICAL FIELD[0001]The present disclosure relates to an interferon-α (IFN-α) fused protein having IFN-α fused to a cytoplasmic transduction peptide (CTP). More particularly, the disclosure relates to a fused protein wherein a CTP, which binds well to cell-membrane barriers and enables translocation into the liver, is fused to a human IFN-α at the N-terminal or C-terminal thereof, thereby enhancing the conjugation capacity of cell membranes, inhibiting CTP transport into the cell nucleus after being introduced into the cell, and enhancing the translocation and settlement of the fused protein into the liver and of transduction to the liver tissue.BACKGROUND ART[0002]Interferons (IFNs) are used to treat hepatitis and marketed as interferon-α (Intron A: Schering, Roferon A: Roche) or PEGylated IFN (PegIntron: Schering, Pegasys: Roche) wherein PEG is added to make the interferon last longer in the body.[0003]The early side effect of interferon-α therapy includes fever, chills, lethargy...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/21C12N15/21C12N15/63A61P35/00C12N1/19C12N5/10C12P21/00A61P1/16C07K14/56C12N1/21
CPCA61K38/00C07K2319/10C07K2319/00C07K14/56A61P1/16A61P35/00
Inventor LEE, CHAN KYUYANG, SEONYOUNGKANG, JURYU, KANGLEE, HYUN SOOBAE, YONG SOO
Owner JW CREAGENE
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