Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Test strip for determining concentration of creatinine in a body fluid sample

a test strip and creatinine technology, applied in the field of test strips for determining the concentration of creatinine in body fluid samples, can solve the problems of short shelf life of nmhase so produced, and the inability to create test strips, etc., and achieve the effect of difficult reproduction

Inactive Publication Date: 2006-10-12
POLYMER TECH SYST
View PDF9 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides an apparatus and method for determining the concentration of creatinine in a body fluid sample using a NMHase-catalyzed reaction. The invention eliminates the complicated stabilization procedures for NMHase and uses readily available Trinder reagents and oxidative couplers. The invention also uses a test strip with a reagent layer impregnated with NMHase having its substrate NMH bound thereto, which produces a colored response that is substantially proportional to the concentration of creatinine over its normal and pathological range. The invention avoids the need for a separate blank reaction adjustment and uses a thresh level of H2O2 to produce color. The invention provides a simpler and more cost-effective method for creatinine testing.

Problems solved by technology

The NMH substrate can be removed, but shelf life of the NMHase so produced is very short, and this NMHase is thus unsuitable for creating a test assay.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Test strip for determining concentration of creatinine in a body fluid sample
  • Test strip for determining concentration of creatinine in a body fluid sample
  • Test strip for determining concentration of creatinine in a body fluid sample

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0048] Blood Separation Membrane

[0049] Ahlstrom Grade 144, which is a glass fiber membrane with a thickness of 0.378 mm, was impregnated with an impregnation solution of the following composition:

Water, D.I. Purified800 g NaCl10 gSorbitol75 gCitric Acid0.21 g  pH 4.2-4.4

Q.S. to 1000 ml with D.I. Water

The membrane was immersed in a re-circulating bath of impregnation solution at a rate of 0.5 ft / min. The membrane was dried by passage through a tunnel with blowing warm air (98° to 106° F.) and low humidity (<5% RH).

example 2

[0050] Auxiliary Reagent Membrane

[0051] Schleicher and Schuell Grade 595 paper, which is a pressed paper with a thickness of 0.180 mm, was impregnated by immersion in the following solution:

Sodium Phosphate Buffer, pH 6.020mMNa2ATP60mMN-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline57mM(MAOS)MgCl25mMAscorbate Oxidase150U / ml

Final Volume is 100 ml made with D.I. Water

Impregnation was done manually and the excess liquid removed by lightly wiping with a rubber squeegee. The membrane was dried at 95°-105° F. in a tunnel with blowing air.

example 3

[0052] Creatinine Detection Membrane with 3-Methyl-2-Benzothiazolinone (MBTH)

[0053] A nylon membrane (Cuno Specialty Membrane Products, membrane BLA 045) was impregnated as in example 2 with an impregnation solution of the composition below. The N-methylHydantoinase (NMHase) used in the following solution was not stabilized and had its substrate, NHM bound thereto.

TAPS Buffer, pH 8.0100mMKCl200mMNH4Cl10mMEDTA Na20.5mMTriton X1000.3%Bovine Serum Albumin (BSA)  1%Sucrose4.5%Dextran Sulfate 500,000 M.W.0.75% Creatinine Deiminase430U / mlN-MethylHydantoinase34U / mlSarcosine Oxidase231U / mlCarbamoyl Sarcosine Hydrolyase92.4U / mlPeroxidase1400U / ml3-Methyl-2-Benzothiazolinone (MBTH)1.8mM

Final Volume is 100 ml made with D.I. Water

The impregnated membrane was dried as in Example 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thickaaaaaaaaaa
pHaaaaaaaaaa
Login to View More

Abstract

Apparatus for determining concentration of creatinine in whole blood or plasma using a 1-methylhydantoinase (NMHase) catalyzed reaction, wherein the invention uses commercially available NMHase and thus eliminates prior art stabilization procedures for NMHase. Conveniently, known Trinder reagents and oxidative couplers are used for the indicator system. It has been found that by judiciously selecting the reagents used for the indicator system and / or varying the amount of enzyme NMHase that is loaded into the assay, the effect of the blank reaction can be minimized in the dynamic range of interest such that concentration of creatinine in normal and pathological levels can be measured directly without having to adjust for a blank reaction caused by bound N-methylhydantoin (NMH).

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application is a divisional of U.S. patent application Ser. No. 10 / 426,236 filed Apr. 3, 2003, which claims priority to U.S. Provisional Patent Application Ser. No. 60 / 376,695, filed May 1, 2002.FIELD OF THE INVENTION [0002] The present invention relates generally to methods and test apparatus for determining concentration of analytes such as creatinine in whole blood or plasma. BACKGROUND [0003] Creatine (C4H9O2N3 or α-methyl guanidine-acetic acid) is a compound present in vertebrate muscle tissue, principally as phosphocreatine. Creatine is synthesized primarily in the liver and also in the pancreas and the kidneys. Creatine helps produce energy needed to contract muscles and it is produced at a relatively constant rate. Creatine is eventually spontaneously degraded into creatinine by muscle and is released into the blood. It is then excreted by the kidneys and removed by the body by glomerular filtration. [0004] The amount of cr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/34C12M1/34G01N21/78C12M1/40G01N33/52G01N33/70
CPCC12Q1/34G01N2333/986G01N33/70G01N33/526
Inventor SHULL, BRUCEMUCHNIK, ALEXANDRA
Owner POLYMER TECH SYST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com