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Method and apparatus for performing a lateral flow assay

a technology of lateral flow and assay, which is applied in the direction of chemistry apparatus and processes, laboratory glassware, instruments, etc., can solve the problems of prone user error of devices for qualitative analysis of assays, lack of sophisticated equipment that performs and analyzes, and assays may turn from negative to positiv

Inactive Publication Date: 2004-01-29
RELIA DIAGNOSTIC SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0011] Another object of the invention is to provide a method and apparatus for precisely controlling the timing of a lateral flow assay for more accurate results.
[0013] Another object of the invention is to provide a method and apparatus to execute an algorithm that accurately analyzes the results of a lateral flow assay performed on a test strip.

Problems solved by technology

However, devices for qualitative analysis of assays are often prone to user error, and lack the accuracy of sophisticated equipment that perform and analyze the assays in hospitals and laboratories.
In some applications, if the user mis-times reading the assay device by even a few minutes, the result of the assay may turn from negative to positive.
Still, other devices fail to sufficiently distinguish positive from negative results.
However, whereas readers may reduce operator subjectivity in reading or interpreting assay results, they do not help to control for or help mitigate other sources of assay variability.

Method used

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  • Method and apparatus for performing a lateral flow assay
  • Method and apparatus for performing a lateral flow assay
  • Method and apparatus for performing a lateral flow assay

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Embodiment Construction

[0030] This application hereby incorporates U.S. Patent Application entitled "Improved Lateral Flow Assays," naming Alan Polito, Richard Thayer, Robert DiNello, and George Sierra as inventors, filed Nov. 23, 1998.

[0031] Now turning to the drawings, FIG. 1 shows a preferred embodiment of this invention to include a rapid assay reader 100 for performing and analyzing lateral flow assays. The reader 100 includes a cartridge receptacle 120 for receiving a cartridge having a test strip. A computer system, such as a processor and memory resources, is contained within the reader 100 to control and analyze the assay. The computer system is coupled to an input device such as a keyboard 130 and an output device such as a display 140 to allow for user interaction in performing the assay. The reader 100 may include a battery and / or may be adapted to couple to an AC power supply. A serial port or any other communication outlet may also be provided to upload or download software to and from the c...

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Abstract

An embodiment of the present invention provides a method for performing a lateral flow assay. The method includes depositing a sample on a test strip at an application region, detecting a first detection signal arising from the test strip in the first detection zone, and generating a baseline for the first measurement zone by interpolating between values of the detection signal outside of the first measurement zone and inside of the first detection zone. The method may include locating a beginning boundary and an ending boundary for the first measurement zone on the test strip. Additional detection zones having measurement zones may also be incorporated with the embodiment.

Description

[0001] 1. Field of the Invention[0002] The present invention pertains to a method and apparatus for performing and analyzing a lateral flow assay. More specifically, the invention provides a method and apparatus for determining the amount of an analyte present in a subject sample.[0003] 2. Description of Related Art[0004] Immunoassay technology provides simple and relatively quick means for determining the presence of analytes in a subject sample. The information provided from immunoassay tests are often critical to patient care. Assays are typically performed to detect the presence of particular analytes, such as antibodies that are present when a human subject has a particular disease or condition. Assays practiced under the known art are numerous, and may include assays for diseases such as H. pylori, AIDS or conditions such as pregnancy.[0005] The advancement of immunoassay technology now allows for assay tests to be performed without the complex and expensive equipment used in ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/543G01N33/558
CPCB01L3/5023G01N33/54386G01N33/54393G01N33/558Y10T436/114998Y10S436/807Y10S436/806G01N33/4875G01N27/227G01N33/54388
Inventor POLITO, ALAN J.THAYER, RICHARD M.DINELLO, ROBERT K.SIERRA, GEORGE H.NIXON, DENNISPHILLIPS, ALANNEUBARTH, STUART
Owner RELIA DIAGNOSTIC SYST
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