Bifidus factor beer

A beer and hop technology, applied in the field of functional beer preparation, can solve the problems of lack of bifidobacteria cell component metabolites, increase the cost of beer, inability to decompose and utilize, etc., so as to enhance the immune function of the body, prevent and treat liver diseases, and strengthen the ecology. effect of effect

Inactive Publication Date: 2010-05-12
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, the bifidofactor oligosaccharide beer of report at present generally is to add functional oligosaccharide (being bifidofactor) at any stage after the wort of beer brewing is boiled generally, and brewer's yeast can not decompose and utilize functional oligosaccharide, so It remains in the liquor in the form of the original body. Although these unused small molecular sugars can improve the taste of beer and endow beer with certain physiological functions, their increased ecological functions are very limited; at the same time, artificially prepared bifido Factors are expensive, directly adding bifidofactors increases the cost of beer, and lacks the bacterial components of bifidobacteria (such as lipoteichoic acid, membrane teichoic acid, etc.) and metabolites, which can exert a powerful effect in the human body. Biological functions (such as anti-tumor, anti-fatigue, anti-aging, anti-infection, anti-ionizing radiation, lowering blood fat, preventing and treating cardiovascular diseases, etc.)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1.1 Raw materials: malt, commercially available rice or corn, granular hops.

[0023] 1.2 strains

[0024] 1.2.1 Bifidobacterium (Bifidobacterium, 86321): After hundreds of generations of oxygen-resistant acclimation, it has a certain tolerance to oxygen, but its biological characteristics remain unchanged. The bacteria are preserved in a refrigerator at 4°C by freeze-drying , take it out and resuscitate just before use.

[0025] 1.2.2 Saccharomyces cerevisiae (CICC1700): purchased from the Microbial Culture Collection Center of China Institute of Food and Fermentation Industry.

[0026] Process flow:

[0027] 2.1 Grinding raw materials

[0028] 2.1.1 Malt humidification and crushing Spray water mist at 60°C on the malt to increase the moisture content of the whole grain of malt by 1.5%. The sprayed water mist is fully mixed with the malt, and the humidification time is 2 minutes.

[0029] 2.1.2 Dry crushing of rice The lower the moisture, the finer the crushing and...

Embodiment 2

[0067] 1.1 Raw materials: malt, commercially available rice or corn, granular hops.

[0068] 1.2 strains

[0069] The fusant obtained by cell fusion of Bifidobacterium and Saccharomyces cerevisiae is used as a fermentation strain after regenerating the cell wall, and the strain is preserved in a refrigerator at 4°C by freeze-drying method, and taken out and resuscitated before use.

[0070] Process flow:

[0071] 2.1 Grinding raw materials

[0072] 2.1.1 Malt humidification and crushing Spray water mist at 60°C on the malt to increase the moisture content of the whole grain of malt by 1.5%. The sprayed water mist is fully mixed with the malt, and the humidification time is 2 minutes.

[0073] 2.1.2 The lower the water content of rice dry crushing, the finer the crushing, the better the effect; rice flour should not be stored for a long time to prevent heating and agglomeration.

[0074] 2.2 Preparation of wort

[0075] 2.2.1 The rice flour is gelatinized at 45°C and the ma...

Embodiment 3

[0093] 1.1 Raw materials: malt, commercially available rice or corn, granular hops.

[0094] 1.2 strains

[0095] The functional gene on the DNA of bifidobacteria was transformed into Saccharomyces cerevisiae through a plasmid vector to construct a genetically engineered strain, which was used as a fermentation strain. The strain was preserved in a refrigerator at 4°C by freeze-drying method, and it was taken out and revived immediately before use.

[0096] Process flow:

[0097] 2.1 Grinding raw materials

[0098] 2.1.1 Malt humidification and crushing Spray water mist at 60°C on the malt to increase the moisture content of the whole grain of malt by 1.5%. The sprayed water mist is fully mixed with the malt, and the humidification time is 2 minutes.

[0099] 2.1.2 The lower the water content of rice dry crushing, the finer the crushing, the better the effect; rice flour should not be stored for a long time to prevent heating and agglomeration.

[0100] 2.2 Preparation of w...

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PUM

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Abstract

The invention discloses an isomaltose beer, which is characterized by the following: adopting malt, rice or corn and hop as raw material; utilizing anaerobic bifidobacteria and aerobic traditional beer fement bacteria to brew; using symbiotic elementary to ferment; or using bifidobacteria and brewed yeast to fuse cell; sieving stable strain with two bacterial biological function as ferment bacteria; or utilizing present molecular biological technique to construct ferment bacteria; integrating physiological effect of bifidobacteria and brewed yeast.

Description

technical field [0001] The invention relates to microbiology, microecology, molecular biology, bioengineering fermentation technology and the like, in particular to the preparation of a functional beer. Background technique [0002] Traditional beer is an alcoholic, carbon dioxide and nutritious refreshment beverage made from malt and hops fermented with Saccharomyces cerevisiae. Beer has been around for thousands of years, and the beer industry is almost as old as the wine industry. It has been an important microbial industry for a long time, especially in recent decades. In China, after 1978, due to the rapid emergence of large breweries with a level of tens of thousands of tons or more in cities, large-scale industrial production has been formed. Not only the small workshop style breweries in the countryside are increasingly losing their important status, but the beer industry's high requirements for brewing technology are also more prominent. The United States is the c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12C12/00C12C11/11
Inventor 张德纯李代昆穆小萍邱建
Owner CHONGQING MEDICAL UNIVERSITY
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