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Process for preparing oat beta-glucans

A technology of glucan and oat, which is applied in the field of preparation of high-efficiency biogenic oat β-glucan, can solve the problems of waste of resources, unclear, insufficient total output, etc., to promote growth, inhibit reproduction, and improve immunity Effect

Inactive Publication Date: 2007-05-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, my country's oat deep processing technology is relatively backward at present, and the oat food variety in the market is single, resulting in less than 4% of the total oat production in my country. This situation not only causes a great waste of resources, but also limits my country's oat industry. further development of
[0003] Studies have shown that oat β-glucan, as a high-quality dietary fiber, has the effects of lowering blood fat and blood sugar, but whether it has a positive effect on intestinal function is still unclear

Method used

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  • Process for preparing oat beta-glucans

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Effect test

Embodiment 1

[0038] Take 10kg of oat bran that has been crushed and passed through a 0.5mm sieve, add 120L of water, stir and mix, adjust the pH value to 9.0, treat it under the condition of microwave power 720W for 9min, heat up to 95°C, add heat-resistant α-amylase 0.18 After 1 L, stir for about 30 minutes until the iodine solution does not turn blue, lower the temperature of the extract to 55°C, adjust the pH of the extract to 4.5, add 0.12 L of liquid glucoamylase, continue to stir for 30 minutes, let stand for 30 minutes to precipitate, and heat up after the enzymolysis is complete. Incubate at 100°C for 10 minutes, cool to room temperature and centrifuge (3000r / min, 20min), collect the supernatant and adjust the pH to neutral, double-concentrate with a falling-film double-effect concentrator, add 55L of absolute ethanol to the suspension Centrifuge the solution (3000r / min, 30min) to recover the precipitate, add 8L of water to the precipitate and stir to dissolve, adjust the pH value t...

Embodiment 2

[0040] Take 15kg of oat bran that has been crushed and passed through a 0.5mm sieve, add 180L of water, stir and mix well, adjust the pH value to 9.0, treat it under the condition of microwave power 720W for 9min, raise the temperature to 90°C, and add 0.27L of heat-resistant α-amylase Stir for about 25 minutes until the iodine solution does not turn blue, lower the temperature of the extract to 60°C, adjust the pH of the extract to 4.5, add 0.18 L of liquid glucoamylase, continue to stir for 15 minutes, let stand for 30 minutes to settle, and heat to Incubate at 100°C for 10min, cool to room temperature and centrifuge (3000r / min, 20min), collect the supernatant and adjust the pH to neutral, concentrate twice with a falling film double-effect concentrator, add 90L of absolute ethanol to the suspension Carry out centrifugation (3000r / min, 30min) to recover the precipitate, add 12L of water to the precipitate and stir to dissolve, adjust the pH value to 6.5, add 12g of β-glucanas...

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Abstract

This inventnion relates to a preparation method for high-efficiency prebiotics oat beta-glucan, belonging to the quintessential processing technologies of oat. The said method includes using oat bran as raw materials, crushing, microwave-assisted-extracting, adding amylase and glucoamylase, isoelectric-point-precipitating, centrifugating and separating, concentrating the supernatant, precipitating with ethanol, centrifugating and collecting the precipitation, solving with water, hydrolyzing with beta-glucanase, and spray-drying process to obtain the high-efficiency prebiotics oat beta-glucan with significantly enhanced intestinal and fecal bifidobacteria and actobacillus value-added effect. The method is scientifically and rationally designed, and of great significance in achieving the comprehensive utilization of resources oat and increasing its added value.

Description

technical field [0001] The invention discloses a preparation method of high-yield biogenic oat β-glucan, which belongs to the technical field of oat deep processing. Background technique [0002] Oatmeal is a grain with both nutritional value and health care function. According to statistics, in 2003 my country's oat planting area was 3.6165 million mu, with an average yield of 204.6 kg per mu and a total output of 740,000 tons. As people pay more and more attention to their own dietary health, the global consumption of oats continues to grow, and there is a great demand for oats in the international market. However, my country's oat deep processing technology is relatively backward at present, and the oat food variety in the market is single, resulting in less than 4% of the total oat production in my country. This situation not only causes a great waste of resources, but also limits my country's oat industry. further development. [0003] Studies have shown that oat β-gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/08C12N9/26
Inventor 姚惠源申瑞玲张晖陈正行郭晓娜
Owner JIANGNAN UNIV
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