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Method of cell transdifferentiation

A cell differentiation, cell technology, applied in biochemical equipment and methods, animal cells, cell culture medium, etc.

Inactive Publication Date: 2007-05-16
KANEKA CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technique induces insulin-producing cells by culturing or introducing genes in the presence of nicotinamide without using the above-mentioned differentiation conversion agent, but it is a technique that ultimately uses undifferentiated cells such as fetal cells or precursor cells

Method used

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  • Method of cell transdifferentiation
  • Method of cell transdifferentiation
  • Method of cell transdifferentiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0054] (1) Method

[0055] Mouse osteoblasts were cultured in αMEM medium (hereinafter, osteoblast medium) supplemented with FBS (final concentration 10%), 5-10 mM β-glycerophosphate, 50 μg / ml ascorbic acid, and 1×Glutamax Strains (MC3T3 / E1 ) were established until they became subconfluent.

[0056] After the cells cultured in the osteoblast medium were washed with DMEM medium, the culture condition of the cultured MC3T3 / E1 was changed from the culture using the osteoblast medium to the addition of FBS (final concentration 10%), 100ng αMEM medium (hereinafter, nerve cell culture medium) with bFGF at 10 ng / ml, FGF-8 at 10 ng / ml, EGF at 10 ng / ml, and BDNF at 10 nm / ml (hereinafter, nerve cell culture medium) was used to observe the morphology by microscopy and protein by immunostaining Expression analysis was used to detect changes in cells over time as a result of media changes.

[0057] (2) Results

[0058] After the MC3T3 / E1 cells cultured in the osteoblast medium were tran...

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Abstract

An object of the present invention is to provide a method for transdifferentiating a mature cell without using a DNA methylating agent or a DNA demethylating agent. The present invention provides a method for transdifferentiating a finally differentiated mature cell having a first differentiation phenotype into a finally differentiated mature cell having a second differentiation phenotype that differs from the above first differentiation phenotype by changing culture conditions for the finally differentiated mature cell having the first differentiation phenotype, wherein culture conditions suitable for culturing the finally differentiated mature cell having the above first differentiation phenotype are changed to culture conditions suitable for culturing the finally differentiated mature cell having the above second differentiation phenotype.

Description

technical field [0001] The present invention relates to a method for cell differentiation transformation. More specifically, it relates to a method for switching cell differentiation by changing the culture environment of terminally differentiated mature cells. The present invention further relates to differentiated cells obtained by the above method. Background technique [0002] In the realization of regenerative medicine or cell medicine, in any field, securing the supply of cells that serve as substrates is a necessary and indispensable issue. So far, various methods such as methods for using stem cells and mature cells have been developed. method. [0003] Currently, pluripotent stem cells represented by embryogenic stem cells (ES cells) and mesenchymal stem cells (MSCs), which are used as the mainstream in this field, have the advantage of pluripotency, but on the contrary, on the basis of pluripotency , there are many problems including difficult to control. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/00C12N5/079
CPCC12N2501/13C12N2506/13C12N2501/119C12N2501/115C12N2501/11C12N5/0018C12N5/0618
Inventor 里村一人
Owner KANEKA CORP
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