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Test paper bar for testing colloidal gold of A-group wheel virus antigen

A group A rotavirus detection test paper technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time, unsuitable for epidemiological investigation and on-site detection, complex operation, etc., to achieve simple operation, Effects that are easy to scale and the results are clear and easy to recognize

Inactive Publication Date: 2007-05-16
BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods are complicated to operate, take a long time, require expensive instruments and equipment, and require professionals to carry out in qualified laboratories, etc., which are not suitable for epidemiological investigations and on-site detection.

Method used

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  • Test paper bar for testing colloidal gold of A-group wheel virus antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Group A Rotavirus Antigen Detection Test Strip (see Figure 1)

[0028] The reaction support is a 6.5cm×0.4cm PCV plate; the absorbent pad is a 2cm×0.4cm oil filter paper; a 1.8cm×0.4cm nitrocellulose membrane is sequentially coated with anti-mouse IgG and group A rotavirus monoclonal antibodies; containing 0.4 cm×0.4cm colloidal gold-labeled group A rotavirus monoclonal antibody glass fiber membrane; gold-labeled antibody protective film is a 2.7cm×0.4cm polyester film; that is, a group A rotavirus antigen detection test strip is formed.

Embodiment 2

[0029] Example 2 Group A Rotavirus Antigen Detection Test Strip (see Figure 1)

[0030] The reaction support is a 6.5cm×0.4cm PCV plate; the absorbent pad is a 2cm×0.4cm oil filter paper; a 1.8cm×0.4cm nitrocellulose membrane is sequentially coated with anti-mouse IgG and group A rotavirus monoclonal antibodies; containing 0.4 cm×0.4cm colloidal gold-labeled group A rotavirus monoclonal antibody glass fiber membrane; gold-labeled antibody protective film is 2.7cm×0.4cm glass fiber; that is, a group A rotavirus antigen detection test strip is formed.

Embodiment 3

[0031] Example 3 Group A Rotavirus Antigen Detection Test Strip (see Figure 1)

[0032] The reaction support is a 6.5cm×0.4cm PCV plate; the absorbent pad is a 2cm×0.4cm oil filter paper; a 1.8cm×0.4cm nitrocellulose membrane is sequentially coated with anti-mouse IgG and group A rotavirus monoclonal antibodies; containing 0.4 cm×0.4cm colloidal gold-labeled group A rotavirus monoclonal antibody glass fiber membrane; gold-labeled antibody protective film is 2.7cm×0.4cm filter paper fiber; that is, a group A rotavirus antigen detection test strip is formed.

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Abstract

This invention provides one test bar, which comprises the following steps: a, covering A set of group wheel virus single clone antibody and IgG two ban NC fiber film; b, comprising glue gold label A set wheel single clone antibody glass fiber film; c, applying film analysis double antibody clamper to test A set wheel virus of feces.

Description

Technical field [0001] The invention belongs to the field of biological detection, and specifically relates to a test strip for detecting group A rotavirus (RV-group A) antigen and its application. Background technique [0002] For the diagnosis of rotavirus (RV) infection, the classic method is virus isolation and a double serum increase of 4 times, which is still the gold standard for clinical diagnosis. The development of modern biotechnology has provided many new detection methods for the diagnosis of RV infection, such as: electron microscopy or immunoelectron microscopy to detect rotavirus particle antigens from fecal extracts; application of enzyme-linked immunosorbent assay (ELISA) and nucleic acid probe technology Detect viral particles and antigens; use polyacrylamide gel electrophoresis (PAGE) to directly detect viral RNA in fecal extracts; use reverse transcription polymerase chain reaction (RT-PCR) to detect rotavirus antigens, etc. However, all of the above methods ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/544
Inventor 刘明
Owner BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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