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Schistosomiasis japonica 36D4 gene clone

A schistosomiasis and gene technology, applied in genetic engineering, plant genetic improvement, DNA preparation, etc., can solve the problems of complex antigenicity of schistosomiasis and difficult problems in the development of schistosomiasis vaccines

Inactive Publication Date: 2007-04-04
中国农业科学院上海家畜寄生虫病研究所 +2
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Problems solved by technology

The morphological and biological changes at different developmental stages are bound to be accompanied by different gene differential expression patterns, which lead to the unique metabolism and development process of schistosomiasis, and also make the antigenicity of schistosomiasis very complicated, so the development of schistosomiasis vaccines become a worldwide problem

Method used

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  • Schistosomiasis japonica 36D4 gene clone
  • Schistosomiasis japonica 36D4 gene clone

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] 1. Construction of Schistosoma japonicum Adult Subtractive Library and cDNA Microarray Analysis

[0015] 1. Materials

[0016] 1.1. Experimental animals: New Zealand white rabbits were purchased from the Experimental Animal Center of the Shanghai Branch of the Chinese Academy of Sciences, weighing 2.5 kg, and used as infection hosts for the Chinese mainland strain of Schistosoma japonicum.

[0017] 1.2. The cercariae of the Anhui strain of Schistosoma japonicum in Mainland China: Provided by the snail room of Shanghai Institute of Livestock Parasitic Diseases, Chinese Academy of Agricultural Sciences.

[0018] 1.3. Plasmid, strain: pCR  2.1-TOPO cloning vector was purchased from Invitrogen Company, and DH5α competent cells were purchased from Biotech Company.

[0019] 1.4. Main reagents and tool enzymes:

[0020] Total RNA extraction reagents TRIzol, GeneRacer TM Kit was purchased from Invitrogen, and the mRNA isolation kit oligotex mRNA midikit was a Qiagen produ...

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Abstract

A method for screening Schistosoma japonicum 36D4 gene sequence and its clone are disclosed. The process is carried out by taking Schistosoma japonicum adult as experimental group, taking worm eggs as driven group, constructing adult degressive plasmid as library, selecting, cloning to make cDNA micro-array, and hybridization analyzing to obtain the final product. It applies inhibited hybridization technology.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the nucleotide sequence of the 36D4 gene of Schistosoma japonicum (Chinese mainland strain) and the cloning of the 36D4 gene of Schistosoma japonicum (Chinese mainland strain). Background technique [0002] Schistosomiasis is a widely distributed and serious zoonotic parasitic disease caused by schistosomiasis infection. It is a serious public health problem that needs to be solved urgently. At present, the main prevention and control strategies for schistosomiasis are large-scale chemotherapy, mollusc control and health education in susceptible areas. However, because therapeutic drugs cannot solve the problem of repeated infection of schistosomiasis, the problem of repeated infection of humans and animals is prominent, especially for this disease. It is difficult to achieve long-term effective control of the disease due to repeated infection of livestock, the main source of infect...

Claims

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Application Information

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IPC IPC(8): C12N15/30C07K14/44C12N15/10
Inventor 苑纯秀林矫矫陆珂冯新港傅志强刘金明蔡幼民
Owner 中国农业科学院上海家畜寄生虫病研究所
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