Flow type cell detection method of aerobic non-oxygen-production photosynthetic heterotrophy bacterium

Abstract

The present invention relates to bacteria detection, and flow cytometer detecting process of aerobic anoxygenic photoheterotrophic bacteria (AAPB). The detection process includes the following steps: adding formaldehyde to the sample and culturing; staining the sample with DAPI, collecting forward and lateral scattered light, DAPI fluorescence signal, chlorophyll fluorescence signal and bacterial chlorophyll-a fluorescence signal and separating AAPB from other planktons by means of the difference of signals; separating planktons from other matters by means of DAPI fluorescence signals; separating panktobacteria from other micro planktons by means of the strength of forward and lateral scattered light; eliminating the influence of other matters on the AAPB detection by means of the strength of chlorophyll fluorescence signal; and separating AAPB from other bacteria by means of bacterial chlorophyll-a and obtaining the accurate number of AAPB.

Description

technical field [0001] The present invention relates to the detection of a kind of bacterium, especially relates to the detection of a type of marine ultramicrobial organisms—Aerobic Anoxyenic Phototrophic Bacteria (AAPB), through combining with flow cytometry, establishing A rapid detection method for aerobic anoxygenic photoheterotrophic bacteria. Background technique [0002] Discovered more than 20 years ago (Shiba T, Simidu U, Taga N. Distribution of aerobic bacteria which containbacteriochlorophyll a. Appl Environ Microbiol, 1979, 38: 43-45) is a class of aerobic non-oxygenous photoheterotrophic bacteria with different Aerobic heterotrophic bacteria function in oxygenic photosynthesis, but until recently their important role in marine ecosystems has not received much attention (Kolber Z S, Plumley F G, Lang A S, et al. Contribution of aerobicphotoheterotrophic bacteria to the carbon cycle in the ocean. Science, 2001, 292: 2492-2495), AAPB, as an important part of the ...

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Problems solved by technology

However, studies have shown that this method also counts cyanobacteria, resulting in significant errors in AAPB quantification, which is more pronounced in sea areas or seasons with high cyanobacteria densities

This may bring errors to the estimation of the global AAPB biomass, and even affect the conclusions or inferences on the role of AAPB in the carbon cycle and other aspects (Zhang Yao, Jiao Nianzhi. A study on the quantitative method of aerobic anoxic photoheterotrophic bacteria .Science Bulletin, 2003, 48(1))

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