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Production of invert syrup from sugarcane juice using immobilized invertase

A technology for inverting syrup and sugarcane juice, which is applied in the production of sucrose, inverting sugar, and purifying by physical methods to achieve the effects of improving production efficiency and prolonging life.

Active Publication Date: 2007-02-14
MONTERREY POLYTECHNIC INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But again, none of these references suggest a method of adapting these techniques for the production of invert syrup from sugarcane juice, much less practice it on an industrial scale

Method used

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  • Production of invert syrup from sugarcane juice using immobilized invertase
  • Production of invert syrup from sugarcane juice using immobilized invertase
  • Production of invert syrup from sugarcane juice using immobilized invertase

Examples

Experimental program
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Effect test

Embodiment 1

[0039] special reference figure 1 , sugarcane was extracted using conventional techniques in extraction stage 1. The raw extract 101 (characteristics are shown in Table 1) was passed through the Quick StanBenchtop system of the QSM-04SAP model produced by A / G Technology at a flow rate of 12.0 L / hour under a maximum pressure drop of 30 psi, which contained a microfiltration unit ( figure 1 2) in , equipped with a 0.2 μm filter membrane, thereby producing microfiltered sugarcane juice 201 with the characteristics shown in the second column of Table 1. Impurities 202 are mainly proteins, peptides, phenols and other colored compounds. Microfiltered sugarcane juice 201 was then subjected to ultrafiltration under a maximum pressure drop of 30 psi using a model QSM-04SAP unit produced by A / G Technology ( figure 1 3) is carried out, thereby producing ultrafiltered sugarcane juice 301. Impurities 302 are mainly proteins, peptides, phenols and other colored compounds. The ultrafilte...

Embodiment 2

[0043] Invertase (Grade VII, Catalog number I 4504, Sigma Aldrich, St. Louis, Missouri) was immobilized on chitosan beads (Chitopearl BCW 3505, Wako Chemicals USA, Inc.) by simple adsorption. The size of the beads is 350-390μm, the ion exchange capacity is 0.2-0.3meg / ml, and the specific surface is 150-200m 2 / g. The chitosan beads were washed 3 times with distilled water and 0.02M sodium acetate solution (pH 4.5). Invertase was added to the chitosan beads at approximately 2,000 enzyme units / g bead, and the resulting suspension was kept under agitation at room temperature for 60 minutes. The solution was filtered and the chitosan beads containing the immobilized invertase were washed once with 0.02M sodium acetate solution (pH 4.5).

[0044] Enzyme immobilized on chitosan beads was used to pack a jacketed column (Type XK-16 Pharmacia) with a length of 20 cm and an inner diameter of 16 mm. The length of the immobilized enzyme support system was adjusted to 120 mm. Zirconium b...

Embodiment 3

[0046] This example is a batch wise simulation of a continuous process of another preferred embodiment of the invention. special reference figure 2 , the sugarcane is extracted in an extraction stage 21 using conventional techniques. In the first filtration stage 22, the raw extract 1001 having the characteristics shown in Table 2 was first passed through a sieve No. 100 (100 mesh) under atmospheric pressure. Relatively coarse solid impurities 2002 were collected by filter and discarded. The first filtered juice 2001 is first pasteurized in step 23 at 61.5° C. for 0.5 hours (or at a higher temperature, such as 72° C. for a shorter time, such as 15 seconds) while evaporating and concentrating to 50° white profit. The pasteurized / concentrated juice 3001 was passed through a microfiltration cartridge 24 (2800 cm 2 , CFP-2E-6A, produced by Amersham Biosciences Buckinghamshire, UK), the filter cartridge has a membrane with a pore size of 0.2 μm and operates at a pressure of 13...

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Abstract

This invention is directed to a novel process in which raw sugarcane juice is first refined through a number of steps and then bioenzymatically converted into refined invert syrup or glucose-fructose concentrated solution using a reactor packed with immobilized invertase. The refining steps preferably include a first filtration (particle filtration or microfiltration) followed by a second filtration (microfiltration or ultrafiltration) and then at least one pass through a column packed with an anionic exchange resin. The refined sugarcane juice can be optionally concentrated and / or pasteurized before passing through a reactor packed with immobilized invertase to convert sucrose to glucose - fructose.

Description

field of invention [0001] The present invention relates to a method for converting sugarcane juice or its concentrate into invert (glucose-fructose) syrup using an immobilized invertase. Background of the invention [0002] Invert syrup is widely used in different parts of the food industry such as soft drinks and bakery due to its sweetness and liquid form. Syrups are preferred over crystalline sugars due to the processing advantages afforded by syrups. Invert syrup is highly demanded in soft drink production due to its sweetness and presence in liquid form and similarity to 42 and 55 (the numbers indicate the weight percent fructose content of the total sugar content) fructose corn syrup. Currently, 60-62% of the total sugar produced worldwide is refined sugar from cane and other non-cane sugar sources. 19.2 million hectares of sugarcane and other non-cane sugar sources are planted each year, producing an average of 142 million tonnes of crystalline sugar. In 2002, Mexi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C13B20/14C13B20/16C13FC13K3/00
CPCC13B20/165A23L1/09C13K3/00C13B20/146A23L29/30
Inventor 塞尔西奥·R.·塞尔纳-萨尔迪维亚马尔科·A.·里托-帕洛马雷斯
Owner MONTERREY POLYTECHNIC INST
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