Production of invert syrup from sugarcane juice using immobilized invertase
A technology for inverting syrup and sugarcane juice, which is applied in the production of sucrose, inverting sugar, and purifying by physical methods to achieve the effects of improving production efficiency and prolonging life.
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Embodiment 1
[0039] special reference figure 1 , sugarcane was extracted using conventional techniques in extraction stage 1. The raw extract 101 (characteristics are shown in Table 1) was passed through the Quick StanBenchtop system of the QSM-04SAP model produced by A / G Technology at a flow rate of 12.0 L / hour under a maximum pressure drop of 30 psi, which contained a microfiltration unit ( figure 1 2) in , equipped with a 0.2 μm filter membrane, thereby producing microfiltered sugarcane juice 201 with the characteristics shown in the second column of Table 1. Impurities 202 are mainly proteins, peptides, phenols and other colored compounds. Microfiltered sugarcane juice 201 was then subjected to ultrafiltration under a maximum pressure drop of 30 psi using a model QSM-04SAP unit produced by A / G Technology ( figure 1 3) is carried out, thereby producing ultrafiltered sugarcane juice 301. Impurities 302 are mainly proteins, peptides, phenols and other colored compounds. The ultrafilte...
Embodiment 2
[0043] Invertase (Grade VII, Catalog number I 4504, Sigma Aldrich, St. Louis, Missouri) was immobilized on chitosan beads (Chitopearl BCW 3505, Wako Chemicals USA, Inc.) by simple adsorption. The size of the beads is 350-390μm, the ion exchange capacity is 0.2-0.3meg / ml, and the specific surface is 150-200m 2 / g. The chitosan beads were washed 3 times with distilled water and 0.02M sodium acetate solution (pH 4.5). Invertase was added to the chitosan beads at approximately 2,000 enzyme units / g bead, and the resulting suspension was kept under agitation at room temperature for 60 minutes. The solution was filtered and the chitosan beads containing the immobilized invertase were washed once with 0.02M sodium acetate solution (pH 4.5).
[0044] Enzyme immobilized on chitosan beads was used to pack a jacketed column (Type XK-16 Pharmacia) with a length of 20 cm and an inner diameter of 16 mm. The length of the immobilized enzyme support system was adjusted to 120 mm. Zirconium b...
Embodiment 3
[0046] This example is a batch wise simulation of a continuous process of another preferred embodiment of the invention. special reference figure 2 , the sugarcane is extracted in an extraction stage 21 using conventional techniques. In the first filtration stage 22, the raw extract 1001 having the characteristics shown in Table 2 was first passed through a sieve No. 100 (100 mesh) under atmospheric pressure. Relatively coarse solid impurities 2002 were collected by filter and discarded. The first filtered juice 2001 is first pasteurized in step 23 at 61.5° C. for 0.5 hours (or at a higher temperature, such as 72° C. for a shorter time, such as 15 seconds) while evaporating and concentrating to 50° white profit. The pasteurized / concentrated juice 3001 was passed through a microfiltration cartridge 24 (2800 cm 2 , CFP-2E-6A, produced by Amersham Biosciences Buckinghamshire, UK), the filter cartridge has a membrane with a pore size of 0.2 μm and operates at a pressure of 13...
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