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Method of measuring hatching rate, slush rate and death rate of brine shrimp simultaneously

A technology of artemia eggs and hatching rate, which is applied in animal husbandry and other fields, can solve problems such as troublesome operation, and achieve the effects of convenient application, avoiding a large amount of loss, and high accuracy

Inactive Publication Date: 2007-01-03
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0006] The above measurement method can only measure 1 to 2 indicators at a time, and when measuring the hatching rate, empty shell rate and dead egg rate of Artemia eggs, the operation is also more troublesome

Method used

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Examples

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Embodiment Construction

[0020] specific implementation plan

[0021] In the following, the determination of Artemia eggs produced by the Yellow Sea Industrial Development Corporation in the laboratory of the Yellow Sea Fisheries Research Institute in May 2000 will be further described as an example.

[0022] 1. Put a piece of white filter paper soaked with seawater (filter paper diameter 11cm) in five transparent glass petri dishes with a diameter of 9 cm, and then add 2 ml of sea water to each petri dish to fully soak the filter paper.

[0023] 2. Put about 30-40 Artemia eggs into the above-mentioned 5 petri dishes respectively, keep the distance between the eggs above 0.5mm, and cover the petri dishes. Then put it into a biochemical incubator at 28° C., control the light intensity at 1500-2000 lux, and incubate for 28 hours.

[0024] Petri dish number

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PUM

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Abstract

The method of measuring hatching rate, ghost rate and death rate of brine shrimp simultaneously includes setting sea water soaked white filter paper inside transparent glass culture dish and setting brine shrimp eggs before covering the dish with cover; hatching inside biochemical incubator under controlled temperature and lighting conditions; counting after hatching the hatched eggs with orange larva beside the egg, the death eggs with complete egg grain and the ghost egg with deformation and without larva beside the egg; and calculating the hatching rate, ghost rate and death rate of brine shrimp. The method is simple, convenient and accurate.

Description

technical field [0001] The invention relates to a method for detecting the quality of Artemia eggs, in particular to a method for simultaneously measuring the hatching rate, empty shell rate and dead egg rate of Artemia eggs. Background technique [0002] With the widespread use of Artemia eggs in the fields of aquaculture and biological research, people pay more and more attention to their quality problems, among which the hatchability, dead egg rate and empty shell rate are important indicators of the quality of Artemia eggs. . The current measurement method is as follows: [0003] (1), determination of Artemia egg hatching rate: adopt counting method: count a certain number of Artemia eggs before hatching, count the number of hatched nauplii after hatching, thereby calculate its hatching rate; or adopt density method: No. The first time is to quantitatively sample the samples of Artemia eggs soaked for 2 hours before hatching to count the number of Artemia eggs, and the...

Claims

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Application Information

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IPC IPC(8): A01K67/00
Inventor 刘淇殷帮忠姚建
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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