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Method and kit for pesticide analysis

A pesticide-level technology, applied in the field of rapid detection kits, can solve the problems of analyte non-specificity, time-consuming, undescribed duration of detection limit of pesticides, etc.

Inactive Publication Date: 2006-12-27
COUNCIL OF SCI & IND RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the patent does not describe the detection limit of the pesticide or the duration required for the analysis
[0008] The prior art methods reported above are time consuming
Furthermore, most known methods are analyte nonspecific and require users to have good technical knowledge

Method used

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  • Method and kit for pesticide analysis
  • Method and kit for pesticide analysis

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Embodiment approach

[0026] Preferred embodiments of the method for detecting the presence of pesticides in water according to the present invention include the following:

[0027](1). The methyl parathion (MP) antibody produced from chicken and purified from egg yolk (IgY-MP) was immobilized on an agarose matrix by reductive amination (Hermanson G.T, A.K Mallia and Smitjh P.K, Immobilized affinity ligand techniques (Academic press, 1992, 69-75), the antibody was added to the packed column for flow ELISA, using different concentrations of MP and MP-HRP conjugates according to the following compound. 2). Pack 150 μl of immobilized antibody into a glass capillary column, and equilibrate with cold PBS (pH7.4, 50 mM) of 10 times the column bed volume. A 50 μl sample of this solution containing the pesticide was passed through the column at a flow rate of 50 μl / min. 3). Continue passing PBS for 5 minutes to wash unbound pesticides. 4). Pass 25 μl of MP-HRP conjugate through the column at a flow rate...

Embodiment

[0031] This example illustrates the invention as figure 2 shown in the process. see figure 2 , 150 μl of the immobilized antibody was loaded into a glass capillary column 6, and equilibrated with cold PBS (pH 7.4, 50 mM) of 10 times the column bed volume. 50 μl of sample solution containing insecticide 2 was passed through column 6 at a flow rate of 50 μl / min. Pass PBS 1 continuously for 5 minutes to wash unbound pesticides. 25 μl of MP-HRP conjugate 3 was passed through column 6 at a flow rate of 50 μl / min. Unbound conjugate was eluted for 15 min using PBS 1 containing 0.1% BSA. Preferably, all reagents mentioned above are passed through the column 6 via the peristaltic pump 5 . Finally, the substrate-containing conjugate is removed from the column for chemiluminescent detection as follows:

[0032] Add 25 μl of urea H to 50 μl of the substrate-containing conjugate obtained above 2 o 2 (10 mM Milli Q solution), and placed at room temperature for 2 min. As in 7, the...

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Abstract

A method for rapid detection of pesticide at ppt level using immunochemiluminescence is diclosed. The method comprises packing immobilized antibodies packed in a glass capillary column, passing a sample solution to be tested for pesticide content through said column so that a pesticide-antibody conjugate is formed, removing said pesticide-antibody conjugate from the column and adding thereto, urea H202 followed by Luminol and K3Fe(CN)6, mixing said solution thoroughly until light is generated, and capturing the light generated by a CCD camera, the intensity of the light produced being directly proportional to the amount of pesticide content in said sample. The present invention also discloses a kit for carrying out the method of the present invention.

Description

field of invention [0001] The invention provides a rapid detection kit for pesticide analysis based on a charge-coupled device. The present invention also provides a method for manufacturing a detection kit for pesticide analysis based on a charge coupled device, and its application for detecting pesticide levels in water, food and the environment. Background of the invention [0002] Environmental protection related to water and agriculture requires urgent attention to human health. Major health problems occurred due to the use of contaminated water and food. Organochlorine and organophosphorus pesticides are used in agriculture in many countries. Due to the increased use of pesticides in agriculture in recent decades, surface water, food ingredients and food products are becoming contaminated with pesticide residues. [0003] Pollution monitoring and protection agencies require fast and sensitive methods for analyzing pollutants. Analytical methods commonly used for th...

Claims

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Application Information

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IPC IPC(8): G01N33/50C12Q1/00G01N33/53
CPCG01N2430/00G01N33/5308
Inventor 姆纳·辛弗·萨库尔奈坎卡特·卡讷什·卡兰斯麦斯奥·阿楠萨拉麦阿·库玛尔班咖劳尔·艾什瓦尔·阿米塔拉尼阿蒂玛尔·帕沙尼蒂如·高帕拉·克瑞什纳·卡兰斯
Owner COUNCIL OF SCI & IND RES
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