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Method for auxiliary screening of high-oil corn and dedicated quantitative character gene locus therefor

A technology for high-oil corn and corn, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of difficulty in tracking the genetic effect of a single QTL, and achieve the effect of a quick selection method

Inactive Publication Date: 2006-12-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many scholars have used traditional markers to conduct similar studies on different organisms, but due to the limitations of the conventional markers used, it is difficult to track the genetic effect of a single QTL

Method used

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  • Method for auxiliary screening of high-oil corn and dedicated quantitative character gene locus therefor
  • Method for auxiliary screening of high-oil corn and dedicated quantitative character gene locus therefor
  • Method for auxiliary screening of high-oil corn and dedicated quantitative character gene locus therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the acquisition of oilc6-m1

[0031] 1. Prepare materials

[0032] The high-oil maize mutant CE03005(♀) with purple stems and white kernels was used as the female parent, and the oil content in the kernels was 8.198%. F of 214 individual plants 2 Collect the leaves of a single plant at the big trumpet stage, put them in a -70°C refrigerator for later use, and harvest F 2 The seeds produced by the plant (F 3 grains) to measure the oil content. from F 1 Randomly select 600 seeds from the seeds and plant them on the farm, and harvest self-bred F 2 The seeds produced (F 3 grains) to measure the oil content. Will F 2 The resulting seeds were planted in the experimental station, complete block design, three repetitions, mixed pollination in the plot, and harvested mixed pollinated F 3 The resulting seeds were tested for their oil content. Determination of grain oil content. The results of the frequency distribution of the grain oil content of this gro...

Embodiment 2

[0043] Example 2, using primers p-bnlg1142, and / or p-umc2313, and / or p-bnlg1188, and / or p-bnlg107 to assist in screening high-oil maize varieties

[0044] 1. The experimental materials include the high-oil parent CE03005 (the oil content of the grain is 8.198%) and the F of the high-oil corn mutant selected from the high-oil offspring. 3∶4 (F 3∶4 Refers to F 3 The kernels on the plant are actually F 4 15 high-oil lines (the oil content of the grains are all greater than 6.3%, and the highest is 9%).

[0045] According to the method of Example 1, the following operations are carried out: the genomic DNA of the above-mentioned corn variety is extracted, and the genomic DNA of the above-mentioned corn variety is used as a template, respectively with P-bnlg1422 (sequence 1 and sequence 2 in the sequence table), p-umc2313 (sequence Sequence 3 and sequence 4 in the list), p-bnlg1188 (sequence 5 and sequence 6 in the sequence listing) and p-bnlg107 (sequence 7 and sequence 8 in th...

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Abstract

The invention discloses an auxiliary screening method and specific quantity property gene position for high-oil maize, which is characterized by the following: adopting gene group DNA of detected maize and high-oil maize gene group DNA of known seed oil content as form; proceeding PCR augmentation through primer p-bnlg1142 and or p-umc2313 and or p-bnlg1188 and or p-bnlg107; setting the detected maize as candidate high-oil maize kind if the band type of detected maize augmentation product is the same as the band type of known seed. The invention can detect generation high-oil individual, which adopts seed color as phenotype mark.

Description

technical field [0001] The invention relates to an auxiliary method for screening high-oil corn and its special quantitative trait gene loci. Background technique [0002] Most of the important agronomic traits, such as yield, quality, ripening period, etc., show the genetic characteristics of quantitative traits, that is, they are affected by many quantitative loci (or loci) (Quantitative Trait Loci, QTLs) and environmental factors. For a long time, quantitative genetics has been based on statistical reasoning, that is, polygenes that control quantitative traits are taken as a whole, and the genetic characteristics of QTLs are analyzed and described through the first-level and second-level statistics of mathematical statistics. However, this cannot determine the number of QTLs controlling quantitative traits, let alone the genetic effects of individual QTLs and their locations on chromosomes. If polygenic traits can be decomposed into several single genetic components, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈绍江韩静
Owner CHINA AGRI UNIV
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