Investigating agent and method of serum sodium ion enzyme method

Abstract

The invention discloses an in-serum sodium ion enzyme method determining reagent and method, finding a relatively good concentration by applying different reagent proportions on complexing ability of crown ether which has characters of cavity ether and sodium ions, and it is the same as the cavity ether in the aspects of sensitivity, accuracy, and precision. And the invention has determining effect similar to the determining effect obtained by the method adopting cavity ether, but the invention is simple and convenient and largely reduces cost.

Description

technical field [0001] The invention belongs to a clinical biochemical analysis method and is used for the determination of sodium ions in serum. Background technique [0002] In biochemical clinical experiments, the determination of serum sodium ions is a routine analysis item in hospitals. The normal value of sodium is 136-145mmol / L, and the common electrolyte disorder in clinical practice is hyponatremia. [0003] The earliest analytical method for sodium ions was flame photometry. The principle of the method is that certain sodium atoms to be tested are excited by heating stimulation and emit light with a characteristic wavelength when returning to the ground state. The characteristic wavelength produced by the excited sodium atoms to be measured in the flame, the intensity of the radiant energy is directly proportional to the number of the excited sodium atoms to be measured in the flame, and the number of the excited sodium atoms to be measured is directly proportion...

AI Technical Summary

Problems solved by technology

The original electrode method not only needs to purchase additional instruments, but also needs to replace the electrodes frequently, and the consumption of electrolyte is relatively fast. These reasons make the electrode method to determine the sodium ion in serum more costly, but the enzymatic method does not have these problems , so the measurement cost is much lower

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