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Method for preparing powdered egg yolk coating agent containing anti-aeromonas hydrophila yolk immunoglobulin

A technology of yolk immunoglobulin and Aeromonas hydrophila, which can be applied in the forming or processing of animal feed, animal feed, additional food elements, etc., and can solve the problems that the immune efficiency has yet to be verified.

Inactive Publication Date: 2006-11-22
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of vaccines to control aquatic animal infectious diseases immune pathways and immune efficiency remains to be verified; Aeromonas hydrophila has natural R plasmids related to the drug resistance of sulfonamides, tetracyclines, streptomycin and chloramphenicol. To improve the disease resistance of farmed animals and increase the safety of animal-sourced food, it is necessary to find non-toxic, non-residue, low-cost and high-efficiency materials for the prevention and control of the disease

Method used

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  • Method for preparing powdered egg yolk coating agent containing anti-aeromonas hydrophila yolk immunoglobulin
  • Method for preparing powdered egg yolk coating agent containing anti-aeromonas hydrophila yolk immunoglobulin
  • Method for preparing powdered egg yolk coating agent containing anti-aeromonas hydrophila yolk immunoglobulin

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Embodiment Construction

[0022] The preparation technology of the coating agent of the present invention will be further described below in conjunction with implementation.

[0023] 1. Collection of egg yolk liquid containing specific antibodies

[0024] 1.1. Collect 100 kilograms of eggs prepared with a certain technique and contain anti-Aeromonas hydrophila yolk antibody with a titer of 1:640 or above.

[0025] 1.2. Remove the shell, shell membrane and egg white of the eggs in a conventional way, collect the egg yolk, and obtain 28 kg of egg yolk liquid from 100 kg of fresh eggs.

[0026] 2. Egg yolk liquid embedding

[0027] 2.1. Mix β-cyclodextrin and water in a ratio of 1:1~2, adjust the pH to 6.5, and stir for 30 minutes with a portable mixer with a rotation speed of 250 rpm.

[0028] 2.2. Slowly add the egg yolk liquid in 1.2 to 2.1 (the ratio of egg yolk liquid to β-cyclodextrin is 2.8-5.6:1), and stir for 30-60 minutes with a portable mixer at a speed of 250 rpm.

[0029] 2.3. Mix starch (...

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Abstract

The present invention discloses preparation process of enveloped hydrophilic aeromonad resisting immunoglobulin yolk powder preparation. The preparation process includes collecting yolk liquid of hydrophilic aeromonad resisting yolk egg of titer in 1 to 640 and over, mixing beta-cyclodextrin and water through stirring to obtain beta-cyclodextrin aqua, adding the yolk liquid slowly into the beta-cyclodextrin aqua with the volume ratio between the yolk liquid and water being 2.8-5.6, mixing with starch with water content lower than 8 % through stirring to obtain mixture, pelletizing the mixture in 60 mesh screen, drying in a horizontal drier and sieving in a 80 mesh sieve. The enveloped preparation product has high bioactivity of immunoglobulin yolk, slow releasing characteristic, less influence of pepsin and good taste.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and relates to a preparation process of feed additives, in particular to a feed additive for aquatic products that can regulate animal growth and immune function, specifically anti-Aeromonas hydrophila yolk immunoglobulin egg yolk powder package Membrane preparation method. Background technique [0002] Aetomonas hydrophila is a short, Gram-negative bacterium that is motile. Widely distributed in nature. More and more evidence shows that Aeromonas hydrophila can cause systemic sepsis or local infection in molluscs, fish, amphibians, reptiles, birds and mammals, often causing a large number of animal deaths . In the late 1980s, in the fish outbreaks in China, the Philippines, Thailand, India and other traditional pond farming countries, the bacterium was considered to be an important pathogen. [0003] In order to prevent and control the occurrence of aeromon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/20A23K20/147A23K20/163A23K40/10A23K40/30
Inventor 李肖梁方维焕
Owner ZHEJIANG UNIV
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