Fermented beverage from fractionated corn
一种发酵原料、玉米的技术,应用在啤酒味饮料领域,能够解决爽口性差、蛋白质分解困难、达不到发酵所需目的量等问题
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Embodiment 1
[0057] Example 1 Preparation of Corn Component Isolates
[0058] After lightly washing the selected corn, it contains 2000ppm SO 2 Soak in an aqueous solution of sulfurous acid for 48 hours. After soaking, wash the corn with water, add an appropriate amount of water, and grind it with a disc grinder or coffee mill. Remove the part of the germ that floats up. Grind the remainder well enough to suspend the contents in water. Filter it with a filter cloth to separate the fiber part and the suspension part. Dry the remaining fiber part with hot air at 120°C until the moisture therein reaches 10% or less than 10%. Use it as a corn fiber component.
[0059] The suspension used as the filtrate will precipitate due to time, so the supernatant is replaced with new water to wash the precipitate (performed twice). Put the washed sediment on a shallow plate and shake it with new water while spreading it. At this time, the substance within 1 / 2 of the distance from the starting poi...
Embodiment 2
[0062] Example 2 Manufacture of starch breakdown product (corn saccharified starch)
[0063] Adjust the pH of the starch component obtained in Example 1 to 6 with calcium hydroxide, add an appropriate amount (0.1%) of heat-resistant α-amylase [(Termanyl) 120L: Novozymes Company], and mix well with 103 °C for 5 minutes. When the temperature dropped to 95°C, it was maintained for 90 minutes. When the temperature dropped to 65°C, 0.1% of Biozyme M5 (AMANO ENZYME INC.) and 0.1% of KLEISTASE P L (Dawa Kasei Co., Ltd.) were added as β-amylase and pullulanase, and stirred for about 20 Hour. It is heated to 80°C or higher to inactivate the enzymes. Filter the sugar solution with diatomaceous earth, desalt it with ion exchange resin, and decolorize and deodorize it with activated carbon to obtain corn saccharified starch solution.
[0064] The obtained suspension of saccharified cornstarch can be used directly or after being appropriately concentrated or diluted. In this embodim...
Embodiment 3
[0065] Example 3 Manufacture of corn protein hydrolyzate
[0066] The protein fraction obtained in Example 1 was suspended in warm water so that the solid content accounted for 30%, and the temperature was raised to 95° C. for 20 minutes for sterilization. When the temperature drops to 50°C, adjust the pH to neutral with sodium hydroxide and hydrochloric acid, add about 0.5% of flavorzyme (Novozymes) and protinFA (Yamato Kasei), react for 20 hours, and the decomposition degree is 20% of free amino acids or greater than 20%. In order to inactivate the enzyme, the temperature was raised to 95°C for 20 minutes. This reaction solution was filtered through celite, and decolorized and deodorized with activated carbon to obtain a zein hydrolyzate.
[0067] The obtained zein hydrolyzate can be used directly or after being appropriately concentrated or diluted. However, in this example, it was concentrated under reduced pressure until the solid content accounted for 30%, and then s...
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