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Artificially cultivating nucleoside active matter in cordycepin and its preparing method and use

A technology of artificial culture and active substances, which is applied in the preparation of sugar derivatives, chemical instruments and methods, and medical preparations containing active ingredients, etc. big problem

Inactive Publication Date: 2006-08-23
于荣敏 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In the 1970s, foreign scientists conducted a lot of research on the anticancer activity of cordycepin, but they gave up because of its excessive cytotoxicity.
[0011] The inventor did not find any reports about the nucleoside active substances in the artificial culture of Cordyceps militaris and its preparation method and use in the literature search process

Method used

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  • Artificially cultivating nucleoside active matter in cordycepin and its preparing method and use
  • Artificially cultivating nucleoside active matter in cordycepin and its preparing method and use
  • Artificially cultivating nucleoside active matter in cordycepin and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Artificially cultivate 240g of dry product of Cordyceps militaris, pulverize, add distilled water according to the ratio of 1:8 (w / w), soak and extract twice in a water bath at 60°C, each time for 2 hours, filter, combine the filtrates, and concentrate the filtrates under reduced pressure to obtain an extract I.

[0024] Extract I was dissolved with 2% dilute hydrochloric acid, filtered to obtain acid solution, and the acid solution was extracted five times with dichloromethane;

[0025] The acid solution after extraction is basified to pH10 with dilute NaOH, then extracted three times with n-butanol, combined with n-butanol extract, concentrated under reduced pressure to obtain the artificially cultivated Cordyceps militaris nucleoside active substances, and its purity has greatly improved improvement.

Embodiment 2

[0027] Cancer suppression rate investigation:

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PUM

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Abstract

The present invention relates to active nucleoside matter from artificially cultivated Cordyceps militaris and its preparation process and use. The preparation process includes crushing dry Cordyceps militaris, hot distillated water extraction, filtering, merging filtrate, decompression concentrating to obtain extractum, dissolving the extractum in acid, filtering to obtain acid solution, extracting the acid solution with dichloro methane, alkalifying the extracted acid solution with NaOH to pH 10, extracting with n-butanol, merging extracted liquid, and decompression concentrating to obtain the active nucleoside matter. Pharmaceutical research shows that the active nucleoside matter has high tumor inhibiting rate on liver cancer of mouse. The active nucleoside matter of the present invention may be prepared into antitumor medicine and has the features of high curative effect, low toxicity, etc.

Description

Technical field: [0001] The invention relates to a nucleoside active substance in artificially cultured Cordyceps militaris (L.) Link, a preparation method and application thereof. Background technique: [0002] Cordyceps militaris (L.) Link], also known as Cordyceps militaris and Cordyceps militaris, belongs to Eumycota, Ascomycotina, Pyrenomycetes, Sphaeriales, and Clavicipitaceae ) Cordyceps (Cordyceps), domestic and foreign scholars believe that the asexual stage of Cordyceps militaris is Cephalosporium (Cephalosporium) or Paecilomyces (Paecilomyces). Cordyceps militaris is a widely distributed species, mainly distributed in China, the United States, Japan, the former Soviet Union, West Germany, Canada, Italy and other countries. my country mainly grows in Jilin, Liaoning, Hebei, Shaanxi, Guangxi, Guangdong, Yunnan, Sichuan, Guizhou, Hubei, Hunan and other provinces and regions. [0003] Cordyceps sinensis generally grows in alpine regions above the snow line at an alt...

Claims

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Application Information

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IPC IPC(8): C07H1/08A61K36/068A61P35/00
Inventor 于荣敏汪宇
Owner 于荣敏
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