High efficiency quick breeding method for cichorium intybus test tube seedling

A chicory and high-efficiency technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of long cultivation period, complicated procedures, and low frequency of plant regeneration, and achieve the effect of reducing cultivation cost and increasing reproduction coefficient.

Inactive Publication Date: 2006-08-23
NORTHWEST UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation cycle is long, the cost is high, the procedure is complicated, the plant regeneration frequency is low, and the mutation is easy to occur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High efficiency quick breeding method for cichorium intybus test tube seedling
  • High efficiency quick breeding method for cichorium intybus test tube seedling
  • High efficiency quick breeding method for cichorium intybus test tube seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047]Embodiment 1: choose the plump chicory seeds, rinse with tap water, soak and shake for 30s with 70% ethanol, 0.1% HgCl 2 The surface was disinfected for 12 minutes, rinsed with sterile water 6 times, inoculated on 1 / 2 MS basic medium, illuminated for 16 hours a day, the light intensity was 20001x, and the culture temperature was 25±2°C.

[0048] Cut the base of chicory aseptic seedlings cultured for 20 days and inoculate them with 2.0mg / L6-BA, 0.5mg / LNAA, 10mg / L AgNO 3 , 100mg / LV B1 , 300mg / L proline and 4% sucrose MS medium, 25±2°C, 2500lux light culture for 7 days, the surface of the explant and the incision will gradually produce green adventitious buds, after 15 days, the buds develop Clustered adventitious buds, the number of adventitious buds formed by each leaf is at most 44, with an average of 36.08 (such as figure 1 shown). Cut off the adventitious buds more than 2cm, transfer to the MS medium that adds 0.5mg / L IBA, produce adventitious roots after 7 days, ca...

Embodiment 2

[0050] Embodiment 2: choose the plump chicory seeds of grain, wash with tap water, soak and shake 30s with 70% ethanol, 0.1%HgCl 2 Disinfect the surface for 12 minutes, rinse with sterile water 6 times, inoculate on 1 / 2 MS basic medium, light for 16 hours a day, light intensity 20001x, and culture temperature 25±2°C.

[0051] Cut the middle and upper part of the leaves of chicory aseptic seedlings cultured for 30 days, and inoculate them in the supplementary 1.5mg / L6-BA, 0.5mg / L NAA, 5mg / L AgNO 3 , 50mg / L V B1 , 300mg / L proline and 4% sucrose MS medium, 25±2°C, 2500lux light culture for 7 days, the surface of the explant and the incision will produce green adventitious buds one after another, about 15 days, the buds develop Clustered adventitious buds, the number of adventitious buds formed by each leaf is at most 35, with an average of 29.06 (such as figure 2 shown). Cut out adventitious buds over 2cm, transfer them to 1 / 2MS medium supplemented with 0.5mg / L IBA, 5-7d will...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The quick breeding method of tissue cultured seedling of chicory has the technological feature of using the leaf of chicory as the explant, and includes the following steps: generating abacterial seedling; direct generation of adventitious bud in excised leaf; and the rooting, hardening off and transplanting of the adventitious bud. The present invention adopts abacterial seedling leaf slice of chicory as the explant, each explant can form 20-40 adventitious buds after culturing for 15-20 days and form 30 stuggy tissue cultured seedlings in 35-40 days, and one abacterial seedling with 5 leaves may be used in extracorporeal culturing to obtain about 500 high quality tissue cultured seedlings in 30 days. Compared with conventional excised plant culture system, the present invention has 25-30 days shortened culture period, over 50 % reduced culture cost and 3-5 times higher propagation coefficient.

Description

technical field [0001] The invention relates to a method for rapid propagation of chicory tissue-cultured seedlings, in particular to a method for rapid propagation of chicory tissue-cultured seedlings through the direct generation of adventitious buds from isolated leaves, and belongs to the field of biological group cultured seedlings. Background technique [0002] Chicory (Cichorium intybus.L) is a perennial herb of the genus Chicory in the Compositae family. It is not only a high-yield and high-quality forage, but also a new high-end characteristic vegetable. In addition, chicory contains inulin, horse chestnut bark, horse chestnut bark, and wild lettuce As a coffee substitute, it has the functions of preventing and treating jaundice hepatitis, cardiovascular disease, osteoporosis, etc. It has the development of feeding, food and medicine. potential. There have been some reports on chicory tissue culture in recent years [(1) Varotts, lucchin M, Parrinip.Immature embryos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00A01H3/00C12N5/04A01G7/00
Inventor 韩晓玲贾敬芬
Owner NORTHWEST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap