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Method for detecting B type hepatitis virus specificity T cell by flow cytometer cell factor detecting method

A technology of flow cytometry and cytokines, which is applied in the direction of measuring devices, instruments, and material analysis through optical means, can solve problems such as unseen, and achieve the effect of low cost and high sensitivity

Inactive Publication Date: 2006-08-02
无锡市传染病医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report on the detection of HBV-specific CTL responses in peripheral blood and liver tissue of patients with chronic hepatitis B (CHB) by flow cytometry cytokine detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take 100 μl of whole blood from a normal control subject, and perform the detection operation according to the above-mentioned determination method in the instruction manual. The measured HBV-specific CTL value was 0.2%.

Embodiment 2

[0040] Take 100 μl of whole blood from patients with acute hepatitis B, and perform detection operations according to the above-mentioned determination method in the instruction manual. The measured HBV-specific CTL value was 1.8%.

Embodiment 3

[0042] Take 100 μl of whole blood from a patient with chronic hepatitis B, and perform the detection operation according to the above-mentioned determination method in the instruction manual. The measured HBV-specific CTL value was 0.8%.

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PUM

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Abstract

The present invention relates to a flow cytometry cell factor detection method for detecting hepatitis B virus specific T cell. Said invention utilizes flow cytometry cell factor detection method to detect whole blood, and adopts the following steps: under the environment of having cell factor blocking agent brefeldin adding stirmulus antigen and synergic stimulant in whole blood to make antigen stimulation, after 6 hr of stimulation, washing, identifying lymphocyte and schizolyzing red blood cell, washing and breaking membrane, finally, using fluorescently-labelled antibody to fluorescently stain the cell factor in the cell, after the staining process is completed, utilizing flow cytometer to make analysis.

Description

technical field [0001] The invention relates to a method for detecting hepatitis B virus-specific T cells by a flow cytometry cytokine detection method, which belongs to the technical field of immunological analysis and detection. Background technique [0002] After hepatitis B virus (HBV) infection, a major factor that determines the severity of the disease and the outcome of the course of the disease is the immune response ability of the body. Among them, hepatitis B virus-specific T cells (cytotoxic Tlympho-cyte, CTL, hereinafter referred to as HBV-specific CTL) in patients play an important function in clearing the virus. CD4 in T cells + Cells can differentiate into specific T helper cells (Th) under antigen stimulation. Th can promote the production of HBV-specific CTLs and play an important role in maintaining the activity of HBV-specific CTLs and producing persistent cellular immunity. CD8 in T cells + Cells can be differentiated into HBV-specific CTLs under antig...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/569G01N21/00
Inventor 裴豪杨小娟钱金娟
Owner 无锡市传染病医院
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