Efficient in vifro culture method of fresh flower lily

A technology for in vitro propagation and fresh cut flowers, applied in horticultural methods, botanical equipment and methods, plant phenotype improvement and other directions, can solve the problems of low reproduction coefficient, unsystematic, long reproduction time, etc.

Inactive Publication Date: 2006-05-17
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +1
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AI Technical Summary

Problems solved by technology

(2) Serious pollution
(3) Low reproduction coefficient
(4) Breeding time is too long
In China, Ruan Shaoning and others used to add a certain amount of BA and GA to the rejuvenation medium of lily bulblets, which can break dormancy and advance the flowering period, but there is no statistical data on the specific growth period.
(5) The transplanting survival rate of tissue culture seedlings is low
Numerous scientific researchers in China use sand, vermiculite, peat, garden soil, coconut bran and other materials as substrates to domesticate and cultivate tissue culture seedlings, but the survival rate is still not ideal.
[0005] To sum up, although Lily Rapid Propagation has done a lot of work at home and abroad, it is not systematic, and there are still many technical problems that need to be solved urgently

Method used

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Embodiment Construction

[0022] The present invention is described in detail below in conjunction with embodiment:

[0023] 1. Material sources: The high-quality oriental hybrid lily bulbs imported from Yunnan and the musk lily bulbs provided by the Horticultural Institute of the Chinese Academy of Tropical Agricultural Sciences were used as initial explants.

[0024] 2. Method:

[0025] (1) Material pretreatment and disinfection treatment ①Take high-quality lily bulbs and store them in 4°C for 40 days to devernalize them; ②After pretreatment, the explants are rinsed with streamlined tap water for 12 hours, and then soaked in 75% alcohol for 30 seconds , 0.1% mercuric chloride with constant agitation for 15 minutes.

[0026] (2) Initial explant bud induction culture: cut the initial explant, that is, the scale leaf, into small pieces of 1 cm square, each piece is cut into 4 small pieces according to its size, and inoculated in MS+BA 1.0mg / L+NAA 0.1 mg / L+30g / L sucrose+7g / L agar, pH=5.8, 24°C, cultiva...

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Abstract

The present invention is two-step explant process for in vitro propagating cut flower lily in high efficiency. After high quality oriental hybrid lily and musk lily is hidden to devernalize, bulb in medium size is selected as initial explant to differentiate directly to proliferate buds. The contagious bud as the secondary explant is proliferated through sound seedling culture, rooting culture, test seedling domestication, transplanting and other steps. The present invention provides effective technological way for providing high quality seedling used in producing cut flower lily and has less problems of seed bulb degeneration, contamination, low propagation speed, etc.

Description

[technical field] [0001] The invention relates to a method for rapidly propagating fine seedlings of fresh-cut lily lily scales, leaves and shortened stems in vitro. [Background technique] [0002] Lily (Lilium spp.) is a perennial bulbous flower with huge flowers, rich colors and beautiful flower posture. It can be used not only as cut flowers, potted flowers, but also in gardens and green spaces. With the popularity of lilies in the fresh cut flower market at home and abroad, the production and consumption of lilies have increased year by year, and the ranking of cut lilies in the Netherlands has risen to fifth place, and they have won the title of king of flower bulbs (Lu Meilian, Xu Xinping, Biotechnology in Lily The application of the above, Zhongkai Agricultural Technology College Journal, 15 (4): 69 ~ 76, 2002). Lily not only has ornamental value, but also has medicinal value of dispelling cough and promoting body fluid, invigorating spleen and replenishing qi, clear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/00A01H3/02
Inventor 刘菊华徐碧玉金志强谭光兰
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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