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Composite bacterial additive of alfalfa ensilage

A technology of additives and compound bacteria, applied in bacteria, microorganism-based methods, preservation methods of animal feed raw materials, etc., can solve the problems of reducing the pH value of silage, affecting fermentation, and no alfalfa silage additives yet.

Inactive Publication Date: 2006-04-12
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to a variety of lactic acid bacteria, the green juice fermentation broth also contains a variety of harmful bacterial species. These bacteria also have the role of initiating factors, inducing the expansion of harmful bacterial populations in the process of alfalfa silage, thereby reducing or slowing down The green juice fermented liquid can better promote the expansion and reproduction of lactic acid bacteria, so that the pH value of the silage can not be sharply reduced in a short period of time during the pre-fermentation period and the early acidification stage of the silage alfalfa. This is the green juice fermented liquid used as a silage additive in alfalfa silage The biggest defect that affects the high-quality fermentation of silage alfalfa and the formation of high-quality silage
In further searches, there is no report on mixing green juice fermented liquid with Streptococcus lactis and Streptococcus faecalis as an alfalfa silage additive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 1. Production of green juice fermentation liquid: (1) Take fresh alfalfa plants, remove about 10cm from the base and top, remove the main branches and thicker branches at the same time, keep the remaining young branches and leaves, and chop them into 3-5cm (2) add distilled water in the ratio of alfalfa (g) and water (ml) 1: 2.5, fully mix, mash with a high-speed tissue grinder, let stand for 1 hour, filter with double-layer gauze, press alfalfa raw material: glucose=10g : Add glucose or the syrup of an equivalent amount of glucose to the filtrate in a ratio of 1 g, fully stir, and ferment for 48 hours in an environment of 30° C. to make a green juice fermentation liquid;

[0012] 2. Isolation and purification of Streptococcus lactis: Dilute the green juice fermentation liquid 100 times, place it in a separate solid medium, medium pH = 5.8, culture at 35°C for 48 hours, select colonies with large calcium-dissolving circles and yellow medium , placed in MRS solid medium ...

Embodiment 2

[0021] 1. Production of green juice fermentation liquid: (1) Take fresh alfalfa plants, remove about 10cm from the base and top, remove the main branches and thicker branches at the same time, keep the remaining young branches and leaves, and chop them into 3-5cm (2) add distilled water in the ratio of alfalfa (g) and water (ml) 1: 2.5, fully mix, mash with a high-speed tissue grinder, let stand for 1 hour, filter with double-layer gauze, press alfalfa raw material: glucose=10g : Add glucose or the syrup of an equivalent amount of glucose to the filtrate in a ratio of 1 g, fully stir, and ferment for 48 hours in an environment of 30° C. to make a green juice fermentation liquid;

[0022] 2. Isolation and purification of Streptococcus lactis: Dilute the green juice fermentation liquid 80 times, put it in the isolated solid medium, the pH of the medium is 6.1, culture at 37°C for 48 hours, and select the colonies with large calcium-dissolving circles and yellow medium , placed o...

Embodiment 3

[0031] 1. Production of green juice fermentation liquid: (1) Take fresh alfalfa plants, remove about 10cm from the base and top, remove the main branches and thicker branches at the same time, keep the remaining young branches and leaves, and chop them into 3-5cm (2) add distilled water in the ratio of alfalfa (g) and water (ml) 1: 2.5, fully mix, mash with a high-speed tissue grinder, let stand for 1 hour, filter with double-layer gauze, press alfalfa raw material: glucose=10g : Add glucose or the syrup of an equivalent amount of glucose to the filtrate in a ratio of 1 g, fully stir, and ferment for 48 hours in an environment of 30° C. to make a green juice fermentation liquid;

[0032] 2. Isolation and purification of Streptococcus lactis: Dilute the green juice fermentation liquid 60 times, place it in a solid medium, medium pH = 6.4, cultivate at 40°C for 48 hours, select colonies with large calcium-dissolving circles and yellow medium, Place in MRS solid medium for purifi...

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PUM

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Abstract

A composite microbial additive for preparing the ensilage of alfalfa is composed of the fermented liquid of green juice and the liquid culture medium MRS containing lactosteptococcus and faecal steptococcus. Its preparing process includes such steps as preparing the fermented liquid of green juice, separating lactosteptococcus, activating lactosteptococcus and faecal steptococcus, and proportional mixing. It can decrease the pH value of ensilage and increase its quality.

Description

technical field [0001] The invention relates to an additive in the field of biotechnology, in particular to a compound bacteria additive for alfalfa silage. Background technique [0002] Alfalfa (Medicago sativa L., hereinafter referred to as alfalfa) is a perennial leguminous forage with rich nutritional value. It is a high-quality forage for herbivores and has the reputation of "the king of forage". For a long time, alfalfa has been preserved mainly by sun-dried hay. The biggest disadvantage of this method is the serious loss of nutrients. In recent years, Canada, Japan and other countries have begun to use the method of silage to preserve alfalfa, which has solved the problem of serious loss of nutrients in the process of alfalfa preservation. Because alfalfa has low soluble sugar content and high buffer energy, it is a legume forage that is difficult to silage, and the direct silage effect is not good, so it is necessary to use additives in the silage fermentation proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K3/03C12R1/46A23K30/18
Inventor 安渊田瑞霞王光文
Owner SHANGHAI JIAO TONG UNIV
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