Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quantitative detection reagent and kit for malt agglutinin receptor on sperm membrane surface

A quantitative detection and lectin technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as inability to clinical diagnosis, and achieve the effect of bright colors, easy interpretation, and intuitive results.

Inactive Publication Date: 2012-02-01
深圳华康生物医学工程有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the main method for detecting malt agglutinin receptors on the surface of sperm membrane is nephelometry, which is limited to laboratory research due to the limitation of experimental conditions and technical reasons, and cannot be really popularized and applied in clinical diagnosis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quantitative detection reagent and kit for malt agglutinin receptor on sperm membrane surface
  • Quantitative detection reagent and kit for malt agglutinin receptor on sperm membrane surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The present invention will be described in further detail below through specific examples.

[0039] A kit for quantitative detection of malt agglutinin receptor on sperm membrane surface, comprising the following components:

[0040] (1) buffer solution, (2) fixative solution (3) washing solution (4) enzyme conjugate (5) chromogenic reagent A (6) chromogenic reagent B (7) special glass slide (4 wells / block)

[0041] The preparation process of each detection reagent is as follows:

[0042] (1), the preparation technology method of buffer solution:

[0043] 1), accurately weigh calcium chloride dihydrate (CaCl 2 2H 2 O) 0.132g, potassium chloride (KCl) 0.2g, potassium dihydrogen phosphate (KH 2 PO 4 )0.2g, disodium hydrogen phosphate (Na 2 HPO 4 ) 1.15g, sodium chloride (NaCl) 8.0g, thimerosal 0.2g, magnesium chloride hexahydrate (MgCl 2 ·6H 2 (0) 0.1g, placed in a beaker, added 800 milliliters of distilled water and stirred to dissolve;

[0044] 2) Adjust the p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a reagent and kit for quantitative detection of malt agglutinin receptors on the surface of sperm membranes, and proposes a corresponding detection method to quantitatively detect malt agglutinin receptors on the surface of human sperm membranes and provide information on the location and quantity of WGA receptors on sperm membranes , directly applied to the clinical diagnosis of blindness, the detection method is easy to operate, batch detection can be carried out, and the detection time is greatly shortened.

Description

【Technical field】: [0001] The invention relates to an immunoenzyme cytochemical staining detection reagent. 【Background technique】: [0002] The malt agglutinin receptor on the sperm membrane surface plays an important role in spermatogenesis, maturation, capacitation and fertilization. Once the specific determinant on the sperm membrane recognizes the sperm receptor on the surface of the zona pellucida of the egg cell, it will trigger a series of chain processes such as acrosome reaction, and finally complete the fertilization. Studies have shown that sperm membrane malt agglutinin (WGA) receptors are closely related to sperm fertilization function, the absence of WGA receptors in ejaculated sperm is one of the causes of infertility, and changes in the location and number of WGA receptors can cause infertility or habitual abortion. Therefore, it can be used as a means of clinical diagnosis of unexplained infertility. However, in the prior art, the main method for detectin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/08G01N33/535
Inventor 傅剑华刘瑜胡家纯何林何小红
Owner 深圳华康生物医学工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com