Cross breeding method among venussear mottles of different geographical population
A hybridization method, the technology of variegated abalone, applied in the field of genetic improvement of variegated abalone, to achieve the effect of promoting synchronous release of gametes and strong operability
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Embodiment 1
[0011] In Japan, the abalone with a size of 2.5-3 cm is gradually lowered from the normal water temperature to the transportation temperature of 18 °C at a cooling rate of 2 °C per hour. After the transportation arrives at the destination, it is placed in seawater at 18°C, and then gradually rises to normal water temperature at a rate of 1°C per hour. The survival rate during transportation reaches 90%. According to the living habits of Japanese variegated abalone, the synchronous ripening culture of parent abalone was carried out, and the bait was fed according to the ratio of 100 Gracilaria, 40 Ulva, 30 Wakame, and 8 Spirulina. Select the Japanese abalone with full gonads, inject 1ml of each head or abdomen with a concentration of 10 -3 mol / L of 5-hydroxytryptamine, ejaculate after 2 to 3 hours. The sperm released from Japanese variegated abalone (male) and the eggs released from Taiwan variegated abalone (female) were mixed according to a certain ratio, and the hybrid offs...
Embodiment 2
[0013] Similar to Example 1, the difference is that the abalone with a size of 2 to 2.5 cm is gradually lowered from the normal water temperature to the transportation temperature of 17° C. at a cooling rate of 3° C. per hour. After the transportation arrives at the destination, put it into seawater at 17°C, and then gradually raise the temperature to normal water temperature at a rate of 0.2°C per hour. When carrying out synchronous maturation-promoting cultivation of parent-aubalone, mix bait and feed according to the ratio of Gracilaria 100, Ulva 30, Undaria pinnatifida 50, and Spirulina 15. Inject 1ml into the head or abdomen of each abalone with a concentration of 1.2×10 -3 mol / L of 5-hydroxytryptamine, ejaculate after 2 to 3 hours.
Embodiment 3
[0015] Similar to Example 1, the difference is that the 3.5-4cm abalone is gradually lowered from the normal water temperature to the transport temperature of 16°C at a cooling rate of 1°C per hour. After the transportation arrives at the destination, put it into seawater at 16°C, and then gradually raise the temperature to normal water temperature at a rate of 0.5°C per hour. When carrying out synchronous maturation-promoting cultivation of parent abalones, feed them with mixed bait according to the proportion of Gracilaria 100, Ulva 60, Undaria pinnatifida 20, and Spirulina 1. Inject 0.8ml into the head or abdomen of each abalone with a concentration of 0.8×10 -3 mol / L of 5-hydroxytryptamine, ejaculate after 2 to 3 hours.
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