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Method for diagnosing non-small cell lung cancers

A non-small cell lung cancer, cell technology, applied in the field of biology, cancer research

Inactive Publication Date: 2005-12-07
ONCOTHERAPY SCI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] It has been repeatedly reported that peptide-stimulated peripheral blood mononuclear cells (PBMCs) from specific healthy donors produced significant levels of IFN-γ in response to the peptide, but hardly HLA-γ in chromium-51 release assays. A24 or -A0201 restriction pattern exerts cytotoxic effect against tumor cells (Kawano et al., Cancer Res 60:3550-8 (2000); Nishizaka et al., Cancer Res 60:4830-7 (2000); Tamura et al., Jpn J Cancer Res 92:762-7(2001))

Method used

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  • Method for diagnosing non-small cell lung cancers
  • Method for diagnosing non-small cell lung cancers
  • Method for diagnosing non-small cell lung cancers

Examples

Experimental program
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Effect test

Embodiment 1

[0273] [Example 1] General method

[0274](1) Patient and tissue samples

[0275] Following the principle of informed consent, primary lung cancer tissues were obtained from 37 patients (15 women and 22 men, 46-79 years old; average age 66.0) who underwent lobectomy. Obtain clinical information from the medical record, and diagnose each tumor according to histopathological subtype and pathologist grade; 22 of the 37 tumors are classified as adenocarcinoma, 14 are SCC, and the remaining 1 is gland Squamous cell carcinoma. According to the UICC TNM classification standard, the clinical stage of each tumor is judged. All samples were immediately frozen and embedded in TissueTek OCT medium (medium) (Sakura, Tokyo, Japan), and then stored at -80°C.

[0276] (2) Laser-capture microdissection (laser-capture microdissection), RNA extraction and base RNA amplification at T7

[0277] Laser-capture microdissection (Kitahara et al., Cancer Res 61: 3544-9 (2001)) was used to selectively coll...

Embodiment 2

[0284] [Example 2] Using clinically relevant expression patterns in non-small cell lung cancer cells to identify genes

[0285] Using the data obtained from the expression profiles of all 37 NSCLC samples, a two-dimensional hierarchical clustering algorithm was applied to analyze the similarity between the samples and genes. When the fluorescence intensity of Cy3- or Cy5- is lower than the cut-off value, such genes are excluded from further analysis, as described above (yanagawa et al., Neoplasia 3:395-401 (2001)), and selected Genes with credible values ​​were obtained in more than 95% of the tested cases. Genes with an observed standard deviation <1.0 are also excluded. The 899 genes that passed through this retention filter paper were further analyzed.

[0286] On the sample axis (horizontal axis), 39 samples from 37 cases (two cases were tested in duplicate to verify the reproducibility and reliability of the experimental method) were divided into 2 main subjects according to ...

Embodiment 3

[0288] [Example 3] Identification and characterization of molecular targets for inhibiting the growth of non-small cell lung cancer cells

[0289] In order to identify and characterize new molecular targets that can regulate the growth, proliferation and survival of cancer cells, antisense S-oligonucleotide technology is used to select target genes as follows.

[0290] (1) Identification of the full-length sequence

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Abstract

Disclosed are methods for detecting non-small cell lung cancer using differentially expressed genes. Furthermore, novel human genes whose expression is elevated in non-small cell lung cancer compared to no-cancerous tissues are provided. Also disclosed are methods of identifying compounds for treating and preventing non-small cell lung cancer.

Description

[0001] This application relates to USSN60 / 414,673 filed on September 30, 2002, USSN60 / 451,374 filed on February 28, 2003, and USSN60 / 466 filed on April 28, 2003, which are hereby incorporated by reference. [0002] Invention field [0003] The present invention relates to the field of biology, and more specifically to the field of cancer research. Specifically, the present invention relates to a diagnostic method for non-small cell lung cancer, and genes whose expression is increased or decreased in such cancerous cells. Background technique [0004] Lung cancer is one of the most common fatal tumors in humans. Many genetic changes related to the occurrence and development of lung cancer have been reported. Although genetic changes can help prognosis and predict the risk of metastasis or response to some treatments, information about a single or a limited number of molecular markers usually does not provide satisfactory results for the clinical diagnosis of non-small cell lung can...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/12G01N33/50C12N15/11A61P35/00A61K39/00
CPCG01N33/57423G01N2800/50C12N15/1135C07K14/47C12Q1/6886G01N33/50C12N2310/11A61K31/7088
Inventor 中村佑辅醍醐弥太郎中鹤修一
Owner ONCOTHERAPY SCI INC
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