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Dendritic cell obtained by antigen pulsing

A technology of dendritic cells and antigens, applied in animal cells, virus antigen components, vertebrate cells, etc., can solve the problems of impact on treatment performance, heavy economic burden, patients cannot produce anti-HBs antibodies, etc., and achieve the effect of improving immune response.

Inactive Publication Date: 2005-10-12
TECHNO NETWORK SHIKOKU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0025] Also, since the HB vaccine does not work on people who are taking immunosuppressants, such as liver transplant subjects, patients cannot produce anti-HBs antibodies
However, in liver transplantation related to hepatitis B, since the maintenance of anti-HBs antibody level has a great influence on the treatment performance, after liver transplantation, patients need to be given anti-HBs human immunoglobulin repeatedly
The frequency of administration needs to be once every two weeks, which brings a heavy financial burden of 2.5 million yen a year

Method used

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  • Dendritic cell obtained by antigen pulsing
  • Dendritic cell obtained by antigen pulsing
  • Dendritic cell obtained by antigen pulsing

Examples

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preparation example Construction

[0118] The preparation method of dendritic cells with HBs antigen presenting ability related to this embodiment includes the step of stimulating dendritic cells with HBs antigen. Accordingly, dendritic cells capable of presenting HBs antigens that are effective in the prevention or treatment of hepatitis B can be obtained.

[0119] The above-mentioned preparation method may further include the step of obtaining the above-mentioned dendritic cells from blood. Accordingly, dendritic cells having HBs antigen-presenting ability according to the present invention can be obtained from blood-derived dendritic cells. In addition, if blood is used, dendritic cells can be obtained by a relatively simple operation using a conventionally known method or the like.

[0120] In addition, the above-mentioned preparation method may further include the step of collecting the above-mentioned blood from an organism.

[0121] The above-mentioned organisms may be humans. Accordingly, dendritic c...

Embodiment 1

[0200] The present invention will be specifically described below based on experimental examples, but the present invention is not limited to this example.

experiment example 1

[0201] (Experimental Example 1) Preparation of Dendritic Cells

[0202] (1) Using a syringe (Terumo Syringe SS-50ESZ: produced by Terumo Co.) moistened with heparin (Novo-heparin Note 1000: produced by Aventis Co.), from each subject (who has experience in repeatedly administering HB vaccine) 5 healthy volunteers) blood collection of 40-60ml. The obtained blood was fully mixed with the same amount of phosphate-buffered saline (PBS) as the blood in a special clean bench. Then, mononuclear cells were separated according to the specific gravity centrifugation method using Ficoll-Conray solution (specific gravity 1.077). Centrifuge at room temperature, 1800rpm-3000rpm for 30 minutes, and take out the middle layer after centrifugation as mononuclear cells.

[0203] (2) At the same time, autoserum was obtained from each subject according to the following steps. First, about 10 ml of blood was collected from each subject using another syringe (Terumo Syringe SS-10ESZ: manufactured...

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Abstract

Dendritic cells effective in the prevention and curing of diseases, especially hepatitis B. Dendritic cells having HBs antigen presenting capability obtained by a process including the step of pulsing dendritic cells with HBs antigen are effective as a dendritic cell vaccine in the prevention and curing of hepatitis B. As the HBs antigen, use can be made of an HBs antigen contained in HB vaccine. Vaccine pulsed dendritic cells obtained by a process including the step of pulsing dendritic cells with a vaccine can be used as a dendritic cell vaccine in not only patients but also healthy subjects. As the vaccine for pulsing, use can be made of a wide variety of vaccines.

Description

technical field [0001] The present invention relates to dendritic cells that can be used for the prevention or treatment of diseases. Background technique [0002] (Dendritic cell vaccine therapy and its problems) [0003] In areas such as cancer treatment, dendritic cell vaccine therapy has attracted attention. [0004] Dendritic cells are antigen-presenting cells (APCs) with the strongest antigen-presenting ability in organisms. By presenting ingested antigen epitopes to T cells to activate T cells, immune responses can be improved. [0005] Dendritic cell vaccine therapy is a therapeutic method in which dendritic cells are stimulated outside the body by using substances extracted from cancer cells or cancer-specific antigens, and antigen presentation ability is improved, and the dendritic cell vaccine is returned to the body as a dendritic cell vaccine. [0006] The substance extracted from cancer cells is generally a mixture of various substances. Therefore, since den...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61K35/14A61K35/15A61K39/29A61P1/16A61P31/12A61P31/20A61P37/04A61P43/00C12N5/07C12N5/0784C12N5/0786
CPCA61K39/292A61K2039/5154A61K39/12A61P1/16A61P31/12A61P31/20A61P37/04A61P43/00A61K39/4622A61K39/461A61K39/464838
Inventor SK.·MD.·法兹勒·阿克巴尔恩地森一
Owner TECHNO NETWORK SHIKOKU
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