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Method for determining separate peak among chromatographic overlapping peaks

An overlapping peak and separate technology, applied in special data processing applications, measuring devices, instruments, etc., can solve problems such as narrow application range, quantitative error, and impact on chromatographic quantitative analysis research

Inactive Publication Date: 2005-06-29
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This overlapping peak due to incomplete separation greatly affects the study of chromatographic quantitative analysis and chromatographic peak shape
In order to overcome this difficulty, the vertical cut (middle cut) method and the tangent method are widely used in quantitative analysis, but the quantitative error caused by it sometimes exceeds 20%.
The solution is mainly based on genetic algorithm, neural network algorithm, etc., which use exponentially modified Gaussian function (EMG) to simulate the peak shape of the chromatogram. Quantitative results and peak shape simulation, narrow range

Method used

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  • Method for determining separate peak among chromatographic overlapping peaks
  • Method for determining separate peak among chromatographic overlapping peaks
  • Method for determining separate peak among chromatographic overlapping peaks

Examples

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Embodiment 1

[0043] Tables 1 and 2 illustrate the quantitative results of the present invention through different calculation examples.

[0044] Tables 1 and 2 are the investigation results of quantitative accuracy of overlapping peaks formed by two individual peaks of different peak shapes with different overlapping degrees using the present invention. Table R s is the separation degree of overlapping peaks, reflecting the size of the overlapping degree of overlapping peaks; a and b are the coefficients of Langmuir adsorption equation q=ap / (1+bp) respectively, and the change of b value reflects the change of chromatographic peak shape; chromatographic components Mass m=q+p, where q and p are the mass of components in stationary phase and mobile phase respectively. First, two separate peaks with different tailing degrees are calculated by plate theory, superimposed to form an overlapping peak, and then the individual peaks are calculated for the overlapping peaks according to the calculat...

Embodiment 2

[0050] operating conditions

[0051] Gas chromatography column: SE-30, 2m×3mm

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Abstract

This invention discloses a method to determine the single peak in the chromatogram-overlapped peak, which belongs to improvement technique of the chromatogram ration and shape analysis. The method is characterized by comprising the following steps: matching and iterating and analogue computation, wherein, the iterate process comprises the identification of the single peak shape, single peak computation parameter determination and the single peak got from first iteration by tower theory; the said analogue computation comprises the multiple process to get the single peak by minus of the overlapped peaks till get the set accuracy of the single peak.

Description

technical field [0001] The present invention relates to a method for determining individual peaks among overlapping peaks in a chromatogram. It belongs to the improved technique of chromatographic quantification and chromatographic peak shape analysis. Background technique [0002] In the existing chromatographic separation and analysis process, it is inevitable that the chromatographic peaks overlap. When the different components in the sample cannot be completely separated in the chromatographic column, the component bands overlap, and when they flow out of the chromatographic column and pass through the detector, they are all detected, resulting in the overlapping of chromatographic peaks. The overlapping peaks caused by incomplete separation greatly affect the research of chromatographic quantitative analysis and chromatographic peak shape. In order to overcome this difficulty, vertical cut (middle cut) method and tangent method are widely used in quantitative analysis...

Claims

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Application Information

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IPC IPC(8): G01N30/86G06F19/00
Inventor 范国梁刘蔚李发光白茂森宋崇林宋威
Owner TIANJIN UNIV
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