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Method for preparation biological bacterium for preventing and curing pseudo-ginseng root rot and its application

A technology of biological bacterial agent and root rot, which is applied in the field of preparation and application of biological bacterial agent for the prevention and treatment of Panax notoginseng root rot, which can solve the problems of pesticide residues, difficulty in large-scale implementation of production, etc.

Inactive Publication Date: 2005-06-29
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the crop rotation period generally requires more than 8 years, and it is difficult to implement it on a large scale in production
Although root irrigation with pesticides is effective, the problem of pesticide residues is serious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the method for small-scale preparation powder

[0047] The specific method of fermenting the bacterial agent in the 20-liter fermenter and the process of preparing the bacterial agent are as follows: put 16 liters of YDFG medium in the fermenter, sterilize it at 121°C for 90 minutes, insert 1 liter of liquid culture bacteria, and ventilate The rate is 0.7, the rotation speed is 450rpm, and the temperature is within the fluctuation range of 28-35°C for 2-4 days. After 1 day of fermentation, the number of bacteria is detected every 4 hours. When the number of bacteria reaches 10 13 Individuals / mL or more, and stop the fermentation in time when the number no longer increases. Then mix according to the ratio of 50 ml of fermentation end product: 45 g of filler: 5 g of auxiliary agent, dry at 35-45°C until the moisture content is less than 5%, and finally seal it with a plastic bag, and store it in a ventilated and dark place at 4°C or room temperature place. ...

Embodiment 2

[0048] Embodiment 2: the method for large-scale preparation powder

[0049] The specific method of fermenting the bacteria in a 4000-liter fermenter and the preparation process of the bacterial agent are as follows: put 3500 liters of YDFG medium in the fermenter, sterilize it at 121 ° C for 120 minutes, insert 175 liters of liquid culture bacteria, and ventilate The rate is 0.9, the rotation speed is 450rpm, and the temperature is within the fluctuation range of 28-35°C for 2-4 days. After 1 day of fermentation, the number of bacteria is detected every 4 hours. When the number of bacteria reaches 10 13 Individuals / mL or more, and stop the fermentation in time when the number no longer increases. Prepare powder by the method among the example 1 afterwards. The percentage by weight of the microorganisms and their metabolites in the prepared powder is 75%.

Embodiment 3

[0050] Embodiment 3: the method for preparing granule

[0051] According to the method of embodiment 1 or embodiment 2, microorganisms and metabolites thereof are produced by fermentation. Mix the end product of fermentation with 25% sodium diatomate solution in a volume ratio of 1:1, then use a granulator to make granules with a diameter of 4mm and a length of 4.5cm, and dry them at 35°C to reach the moisture content Less than 5%, made into granules. The weight percentage of the microorganisms and their metabolites in the prepared granules is 45%.

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Abstract

The invention relates to a method for preparation biological bacterium for preventing and curing pseudo-ginseng root rot and its application, wherein the preparation process comprises the steps of microbiological bacterial culture, microorganism mass propagation and bacterium fabrication. The microorganism employed is Brevibacillus laterosporus G4 bacterial strain, which was preserved in Wuhan Univ's CCTCC with the collection number of CCTCC No:M203045.

Description

Technical field: [0001] The invention relates to a preparation method and application of a biological fungicide for preventing and treating Panax notoginseng root rot, which belongs to microbial pesticides [0002] technology field. Background technique: [0003] Sanqi (Panax notoginseng (Burk.) F.H.Chen, 1948), also known as Tianqi, Shanqi and Jinbuhuan, etc., is a perennial shade herbaceous plant of the Araliaceae genus Panax, which is a remnant of the Tertiary ancient tropical The species only exists in some middle and high altitude areas of Wenshan Prefecture in Yunnan Province and Baise area in Guangxi Autonomous Region near 23°30′N latitude. Due to its multiple effects of promoting blood circulation, reducing swelling, relieving pain, and nourishing and strengthening the body, it is widely used as a precious medicinal material in Chinese medicine in my country for the prevention and treatment of various diseases, especially the increasingly serious cardiovascular dise...

Claims

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Application Information

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IPC IPC(8): A01N63/04
Inventor 莫明和张克勤黄英周薇田宝玉
Owner YUNNAN UNIV
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