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Method for identifying fast-growing fish in different salinity

A technology of salt concentration and fish, which is applied in the direction of biochemical equipment and methods, fish farming, microbial measurement/inspection, etc., can solve the problems of inability to identify tilapia strains, etc.

Inactive Publication Date: 2005-02-02
UNIVERSITY OF NEW HAMPSHIRE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, apart from trial and error, current methods cannot identify tilapia strains grown in water with different salinity concentrations

Method used

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  • Method for identifying fast-growing fish in different salinity
  • Method for identifying fast-growing fish in different salinity
  • Method for identifying fast-growing fish in different salinity

Examples

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Embodiment Construction

[0025] The most basic embodiment of the present invention is a kind of fish that can be selected according to the prolactin genotype of the fish (tilapia is preferred), and then raised and reproduced in a saline environment compatible with its prolactin genotype to obtain the best method of growth.

[0026] As noted, past experiments suggested that mRNA levels of prolactin 1 and 2, as well as serum prolactin levels, decreased when tilapia were moved to a saltier environment, with this effect being more pronounced for prolactin 1. Therefore, the applicant's experiments and examples all refer to prolactin 1, but the method of the present invention is not limited to prolactin 1 only.

[0027] At the time of development of the present invention, applicants initially focused on the microsatellite closest to the start of the prl1 transcribed region (approximately 200 base pairs bp). Applicants mated Oreochromis (saltwater adapted) females from Mozambique with Nile Oreochromis (fres...

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Abstract

Methods of selecting fish for breeding and optimum growth in conditions of various salinity are disclosed. Fish are selected for growth in specific salinity based on their prolactin 1genotype. A simple sequence repeat polymorphism (microsatellite) in the tilapia prolactin (prl 1) promoter is associated with differences in prl 1 expression and differences in growth in 'salt-challenged' or 'salt-effected' fishes. This discovery suggests that dinucleotide microsatellites may represent an under-appreciated source of genetic variation for regulatory evolution, and belie the textbook interpretation that non-coding microsatellite length variation lacks functional consequences. Thus, the methods of the invention include determining or selecting the salinity of the environment in which the fish will be grown; determining the prolactin genotype of at least one male and at least one female fish being considered for breeding; breeding male and female fish having the desired genotype(s) to result in offspring having known, predictable genotypes; and raising the fish in a salinity environment compatible with the fishes' genotype(s).

Description

[0001] related application [0002] This application claims the benefit of Patent Application Serial No. 60 / 315.156, filed August 27,2001. [0003] Government funding [0004] This invention was supported in part by NSF DBI 9803845, USDN / NRICGP 00352059287. [0005] copyright [0006] Portions of the disclosure of this patent document include material that is claimed to be copyrighted. The copyright owner has no objection to the facsimile reproduction by anyone of the patent document or the patent disclosure as it appears in the USPTO patent literature, but all copyright rights are reserved whatsoever. field of invention [0007] The present invention relates to the selection of fish for optimum growth. More specifically, the present invention relates to methods for the selection of fish species, in particular tilapia, which grow optimally in water of varying salinity. Most particularly, the present invention relates to the use of polymorphisms in the tilapia prolactin ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K61/10A01K67/02C12Q1/68C12Q1/6888
CPCA01K67/02A01K61/00C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/158A01K61/10Y02A40/81
Inventor J·斯特利尔曼T·科切
Owner UNIVERSITY OF NEW HAMPSHIRE
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