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Electrochemical biosensor

一种生物传感器、电化学的技术，应用在电化学生物传感器领域，能够解决错误判断、付出生命的代价、大量损耗等问题

Inactive Publication Date: 2005-02-02
ISENSE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However the disclosed method cannot be achieved by simple dispersion of the reagent mixture on the working electrode, but requires additional steps in the fabrication process or substantial loss of reagents in the printed reagent layer.
[0007] For those who routinely monitor blood glucose levels with disposable biosensor strips, the bias that relies on large numbers of blood cells can lead to false positives and even cost lives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the preparation of the reagent layer composition solution not containing fatty acid

[0053] A mixture containing 30 mg of hexaamine ruthenium(III) chloride (41.6% by weight), 1 mg of carboxymethylcellulose (1.4% by weight), 1 mg of Triton X-100 (1.4% by weight), and A mixture of 40 mg of glucose oxidase (55.6% by weight) was dissolved in 1 ml of PBS buffer solution (pH 6.5) (pH 6.4), and the remaining particles in the solution were filtered off. Place the reagent solution in the syringe of the pneumatic dispenser (EFDXL100).

Embodiment 2

[0054] Embodiment 2: Preparation of the reagent layer composition solution comprising fatty acid

[0055] Containing 30 mg of hexaamine ruthenium(III) chloride (32.6% by weight), 1 mg of carboxymethylcellulose (0.8% by weight), 5 mg of polyvinylpyrrolidone (4% by weight), 1 mg of trinitrotoluene X- 100 (0.8 wt%), 20 mg of lauric acid (15.7 wt %), 30 mg of myristyltrimethylammonium bromide (23.6 wt %), and a mixture of 40 mg of glucose oxidase (31.5 wt %) were dissolved in 1 ml PBS buffer (pH 6.4), and filter the remaining particles in the solution. The reagent solution was placed in the syringe of the pneumatic dispenser (EFD XL100).

Embodiment 3

[0056] Example 3: Preparation of reverse type two-electrode biosensor

[0057] Such as figure 2 As shown, the working electrode 104 and electrode connector 106 were screen printed with conductive carbon paste and cured at 140° C. for 5 minutes. Then, a silver paste is used to screen print the circuit connector on one end of the electrode connector 106 . The upper substrate with the printed electrode as reference (auxiliary) electrode 105 was screen printed with carbon paste and cured. Finally, the end of the reference electrode 105 was screen-printed with silver paste to form a circuit connector to prepare a biosensor.

[0058] The sampling spacer 200 including the sampling slot 101, the exhaust pipe 102 and the void 103 was placed on the lower substrate by pressing a double-sided adhesive tape made of polyester. The ratio of the width of the exhaust pipe 102 to the width of the sampling tank 101 is 2:1, and the total amount of blood sample in the sampling part 100 is adju...

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PUM

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Abstract

There is provided the reagent layer composition that can substantially reduce the measurement bias arising from hematocrits. The addition of fatty acid (4-20 carbons) and quaternary ammonium salt to a commonly used reagent layer composition composed of an enzyme, an electron transfer mediator, and several water soluble polymers not only reduce the hematocrit level-dependent bias but also provide very stable performance for an extended period of time. Disclosed are also various types of sub microliter sample volume electrochemical biosensors that are suitable to use with the reagent layer composition of present invention.

Description

technical field [0001] The present invention relates to electrochemical biosensors. More specifically, the present invention relates to an electrochemical biosensor comprising a reagent layer composition that results in a dramatic reduction in measurement bias caused by hematocrits. The reagent layer composition contains enzyme, electron transfer mediator, water-soluble polymer, fatty acid (alkyl chain length: 4-20 carbon atoms), and quaternary ammonium salt. Various types of sub-microliter sample volume electrochemical biosensors suitable for use with the reagent layer compositions of the present invention are also disclosed. Background technique [0002] The diagnosis and prevention of diabetes requires regular monitoring of blood sugar levels. This is easily done by using strip biosensors designed for hand-held readout devices alone. Many commercially available biosensors quantify the amount of glucose in a total blood sample using the following response: [0003] &l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327C12Q1/00C12Q1/25G01N27/416
CPCC12Q1/004C12Q1/001C12Q1/25
Inventor 崔刚俞在炫金文焕金周勇严正熙南学铉车根植
Owner ISENSE CORP
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